RRC ID 1784
著者 Oshima K, Takeda M, Kuranaga E, Ueda R, Aigaki T, Miura M, Hayashi S.
タイトル IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis.
ジャーナル Curr Biol
Abstract Differentiated cells assume complex shapes through polarized cell migration and growth. These processes require the restricted organization of the actin cytoskeleton at limited subcellular regions. IKK epsilon is a member of the IkappaB kinase family, and its developmental role has not been clear. Drosophila IKK epsilon was localized to the ruffling membrane of cultured cells and was required for F actin turnover at the cell margin. In IKK epsilon mutants, tracheal terminal cells, bristles, and arista laterals, which require accurate F actin assembly for their polarized elongation, all exhibited aberrantly branched morphology. These phenotypes were sensitive to a change in the dosage of Drosophila inhibitor of apoptosis protein 1 (DIAP1) and the caspase DRONC without apparent change in cell viability. In contrast to this, hyperactivation of IKK epsilon destabilized F actin-based structures. Expression of a dominant-negative form of IKK epsilon increased the amount of DIAP1. The results suggest that at the physiological level, IKK epsilon acts as a negative regulator of F actin assembly and maintains the fidelity of polarized elongation during cell morphogenesis. This IKK epsilon function involves the negative regulation of the nonapoptotic activity of DIAP1.
巻・号 16(15)
ページ 1531-7
公開日 2006-8-8
DOI 10.1016/j.cub.2006.06.032
PII S0960-9822(06)01750-7
PMID 16887350
MeSH Actins / metabolism* Animals Apoptosis / physiology* Blotting, Western Cell Differentiation / physiology* Drosophila / physiology* Drosophila Proteins / metabolism* Genetic Testing Histocytochemistry I-kappa B Kinase / metabolism* Inhibitor of Apoptosis Proteins / metabolism* Microscopy, Fluorescence Morphogenesis / physiology* Trachea / cytology Trachea / growth & development
IF 9.601
引用数 73
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
遺伝子材料 pUAST-IKKepsilon (RDB08029) pUAST-IKKepsilonK41A (RDB08030)
ショウジョウバエ 2615R-1 2615R-2 12284R-2 8091R-1 8091R-2 DGRC#109662