RRC ID 21953
著者 Ruben M, Drapeau MD, Mizrak D, Blau J.
タイトル A mechanism for circadian control of pacemaker neuron excitability.
ジャーナル J Biol Rhythms
Abstract Although the intracellular molecular clocks that regulate circadian (~24 h) behavioral rhythms are well understood, it remains unclear how molecular clock information is transduced into rhythmic neuronal activity that in turn drives behavioral rhythms. To identify potential clock outputs, the authors generated expression profiles from a homogeneous population of purified pacemaker neurons (LN(v)s) from wild-type and clock mutant Drosophila. They identified a group of genes with enriched expression in LN(v)s and a second group of genes rhythmically expressed in LN(v)s in a clock-dependent manner. Only 10 genes fell into both groups: 4 core clock genes, including period (per) and timeless (tim), and 6 genes previously unstudied in circadian rhythms. The authors focused on one of these 6 genes, Ir, which encodes an inward rectifier K(+) channel likely to regulate resting membrane potential, whose expression peaks around dusk. Reducing Ir expression in LN(v)s increased larval light avoidance and lengthened the period of adult locomotor rhythms, consistent with increased LN(v) excitability. In contrast, increased Ir expression made many adult flies arrhythmic and dampened PER protein oscillations. The authors propose that rhythmic Ir expression contributes to daily rhythms in LN(v) neuronal activity, which in turn feed back to regulate molecular clock oscillations.
巻・号 27(5)
ページ 353-64
公開日 2012-10-1
DOI 10.1177/0748730412455918
PII 27/5/353
PMID 23010658
PMC PMC4019749
MeSH ARNTL Transcription Factors / biosynthesis Animals Basic-Leucine Zipper Transcription Factors / biosynthesis Behavior, Animal / physiology Circadian Rhythm* / genetics Cryptochromes / biosynthesis Darkness Drosophila / metabolism Drosophila Proteins / biosynthesis Eye Proteins / biosynthesis Larva Light Signal Transduction Neurons / physiology* Period Circadian Proteins / biosynthesis Transcriptome
IF 3.122
引用数 25
WOS 分野 BIOLOGY PHYSIOLOGY
リソース情報
ショウジョウバエ NP2554 (DGRC#104237) 6747R-2 6747R-4