RRC ID 28865
著者 Zhang C, Hong Z, Ma W, Ma D, Qian Y, Xie W, Tie F, Fang M.
タイトル Drosophila UTX coordinates with p53 to regulate ku80 expression in response to DNA damage.
ジャーナル PLoS One
Abstract UTX is known as a general factor that activates gene transcription during development. Here, we demonstrate an additional essential role of UTX in the DNA damage response, in which it upregulates the expression of ku80 in Drosophila, both in cultured cells and in third instar larvae. We further showed that UTX mediates the expression of ku80 by the demethylation of H3K27me3 at the ku80 promoter upon exposure to ionizing radiation (IR) in a p53-dependent manner. UTX interacts physically with p53, and both UTX and p53 are recruited to the ku80 promoter following IR exposure in an interdependent manner. In contrast, the loss of utx has little impact on the expression of ku70, mre11, hid and reaper, suggesting the specific regulation of ku80 expression by UTX. Thus, our findings further elucidate the molecular function of UTX.
巻・号 8(11)
ページ e78652
公開日 2013-11-12
DOI 10.1371/journal.pone.0078652
PII PONE-D-13-18188
PMID 24265704
PMC PMC3827076
MeSH Animals Antigens, Nuclear / genetics* Base Sequence Cell Survival / genetics Cell Survival / radiation effects DNA Damage* DNA Repair / genetics DNA Repair / radiation effects DNA-Binding Proteins / genetics* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism* Drosophila melanogaster / radiation effects Gene Expression Regulation / genetics* Gene Expression Regulation / radiation effects Histones / metabolism Ku Autoantigen Methylation / radiation effects Oxidoreductases, N-Demethylating / genetics Oxidoreductases, N-Demethylating / metabolism* Promoter Regions, Genetic / genetics Promoter Regions, Genetic / radiation effects Tumor Suppressor Protein p53 / metabolism* Up-Regulation / genetics Up-Regulation / radiation effects
IF 2.74
引用数 10
WOS 分野 MULTIDISCIPLINARY SCIENCES
リソース情報
ショウジョウバエ DGRC#202656