RRC ID 30426
著者 Adachi-Yamada T, Nakamura M, Irie K, Tomoyasu Y, Sano Y, Mori E, Goto S, Ueno N, Nishida Y, Matsumoto K.
タイトル p38 mitogen-activated protein kinase can be involved in transforming growth factor beta superfamily signal transduction in Drosophila wing morphogenesis.
ジャーナル Mol Cell Biol
Abstract p38 mitogen-activated protein kinase (p38) has been extensively studied as a stress-responsive kinase, but its role in development remains unknown. The fruit fly, Drosophila melanogaster, has two p38 genes, D-p38a and D-p38b. To elucidate the developmental function of the Drosophila p38's, we used various genetic and pharmacological manipulations to interfere with their functions: expression of a dominant-negative form of D-p38b, expression of antisense D-p38b RNA, reduction of the D-p38 gene dosage, and treatment with the p38 inhibitor SB203580. Expression of a dominant-negative D-p38b in the wing imaginal disc caused a decapentaplegic (dpp)-like phenotype and enhanced the phenotype of a dpp mutant. Dpp is a secretory ligand belonging to the transforming growth factor beta superfamily which triggers various morphogenetic processes through interaction with the receptor Thick veins (Tkv). Inhibition of D-p38b function also caused the suppression of the wing phenotype induced by constitutively active Tkv (TkvCA). Mosaic analysis revealed that D-p38b regulates the Tkv-dependent transcription of the optomotor-blind (omb) gene in non-Dpp-producing cells, indicating that the site of D-p38b action is downstream of Tkv. Furthermore, forced expression of TkvCA induced an increase in the phosphorylated active form(s) of D-p38(s). These results demonstrate that p38, in addition to its role as a transducer of emergency stress signaling, may function to modulate Dpp signaling.
巻・号 19(3)
ページ 2322-9
公開日 1999-3-1
DOI 10.1128/MCB.19.3.2322
PMID 10022918
PMC PMC84024
MeSH Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinases / genetics Calcium-Calmodulin-Dependent Protein Kinases / metabolism* DNA-Binding Proteins / genetics Drosophila Proteins* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Insect Proteins / genetics Insect Proteins / metabolism* Mice Mice, Transgenic Mitogen-Activated Protein Kinases* Molecular Sequence Data Morphogenesis Nerve Tissue Proteins / genetics Phenotype Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Signal Transduction* T-Box Domain Proteins* Transcription, Genetic Transforming Growth Factor beta / genetics Transforming Growth Factor beta / metabolism* Wings, Animal / growth & development p38 Mitogen-Activated Protein Kinases
IF 3.611
引用数 132
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ DGRC#109630