RRC ID 30448
著者 Sano H, Nakamura A, Kobayashi S.
タイトル Identification of a transcriptional regulatory region for germline-specific expression of vasa gene in Drosophila melanogaster.
ジャーナル Mech Dev
Abstract The vasa gene (vas) is essential for germline development in Drosophila melanogaster. Zygotic vas is expressed in pole cells earlier than any other pole cell-expressing genes thus far identified, and VAS protein is continuously present in germline cells throughout development. Here, we report the identification of a regulatory region that directs germline-specific vas expression. A genomic fragment containing the vas locus was linked to enhanced green fluorescent protein (egfp)-vas fusion gene, and the resulting gene was introduced into fly genome. Developmental vas expression was assessed by monitoring the expression of EGFP-VAS in these transformants. The spatio-temporal expression pattern of EGFP-VAS is essentially identical to that of endogenous VAS throughout germline development. By dissecting the vas promoter, we identified a 40-bp regulatory element, which is necessary and sufficient for germline-specific expression during oogenesis. This region interacts specifically with ovarian protein(s). Furthermore, this region is also required for vas expression in pole cells during embryogenesis. These results suggest that a similar mechanism regulates vas expression both in oogenesis and embryogenesis.
巻・号 112(1-2)
ページ 129-39
公開日 2002-3-1
DOI 10.1016/s0925-4773(01)00654-2
PII S0925477301006542
PMID 11850184
MeSH 5' Untranslated Regions Animals Base Sequence DEAD-box RNA Helicases Drosophila Proteins Drosophila melanogaster Female Gene Deletion Gene Expression Regulation, Developmental Green Fluorescent Proteins Immunohistochemistry Luminescent Proteins / metabolism Microscopy, Fluorescence Models, Genetic Molecular Sequence Data Ovary / metabolism RNA Helicases / genetics* Recombinant Fusion Proteins / metabolism Transcription, Genetic* beta-Galactosidase / metabolism
IF 2.126
引用数 64
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ DGRC#109171