RRC ID 30469
著者 Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
タイトル piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
ジャーナル Dev Cell
Abstract Developmental axon pruning is widely used to refine neural circuits. We performed a mosaic screen to identify mutations affecting axon pruning of Drosophila mushroom body gamma neurons. We constructed a modified piggyBac vector with improved mutagenicity and generated insertions in >2000 genes. We identified two cohesin subunits (SMC1 and SA) as being essential for axon pruning. The cohesin complex maintains sister-chromatid cohesion during cell division in eukaryotes. However, we show that the pruning phenotype in SMC1(-/-) clones is rescued by expressing SMC1 in neurons, revealing a postmitotic function. SMC1(-/-) clones exhibit reduced levels of the ecdysone receptor EcR-B1, a key regulator of axon pruning. The pruning phenotype is significantly suppressed by overexpressing EcR-B1 and is enhanced by a reduced dose of EcR, supporting a causal relationship. We also demonstrate a postmitotic role for SMC1 in dendrite targeting of olfactory projection neurons. We suggest that cohesin regulates diverse aspects of neuronal morphogenesis.
巻・号 14(2)
ページ 227-38
公開日 2008-2-1
DOI 10.1016/j.devcel.2007.11.001
PII S1534-5807(07)00419-4
PMID 18267091
PMC PMC2268086
MeSH Alleles Animals Axons / metabolism* Cell Cycle Proteins / metabolism* Cell Proliferation Chromosomal Proteins, Non-Histone / metabolism* DNA Transposable Elements / genetics* Dendrites / metabolism Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / growth & development* Genetic Markers Mitosis* Mosaicism* Mushroom Bodies / cytology Mutagenesis, Insertional Mutation / genetics Nuclear Proteins / metabolism* Olfactory Pathways / metabolism Phenotype Receptors, Steroid / metabolism Transgenes
IF 10.092
引用数 167
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ DGRC#140001