論文 - 詳細
RRC ID | 32432 |
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著者 | Joshi SS, Cheong H, Meller VH. |
タイトル | A strategy for generation and balancing of autosome: Y chromosome translocations. |
ジャーナル | Fly (Austin) |
Abstract |
We describe a method for generation and maintenance of translocations that move large autosomal segments onto the Y chromosome. Using this strategy we produced ( 2;Y) translocations that relocate between 1.5 and 4.8 Mb of the 2nd chromosome.. All translocations were easily balanced over a male-specific lethal 1 (msl-1) mutant chromosome. Both halves of the translocation carry visible markers, as well as P-element ends that enable molecular confirmation. Halves of these translocations can be separated to produce offspring with duplications and with lethal second chromosome deficiencies . Such large deficiencies are otherwise tedious to generate and maintain. |
巻・号 | 8(1) |
ページ | 58-62 |
公開日 | 2014-1-16 |
DOI | 10.4161/fly.27814 |
PII | 27814 |
PMID | 24646827 |
PMC | PMC3974896 |
MeSH | Animals Chromosomes, Insect* Drosophila / genetics* Female Genetic Techniques* Male Translocation, Genetic* Y Chromosome* |
IF | 1.204 |
引用数 | 1 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ショウジョウバエ | DGRC#125639 DGRC#124255 DGRC#124291 DGRC#124216 DGRC#123609 |