論文 - 詳細
RRC ID | 32531 |
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著者 | Yi W, Zhang Y, Tian Y, Guo J, Li Y, Guo A. |
タイトル | A subset of cholinergic mushroom body neurons requires Go signaling to regulate sleep in Drosophila. |
ジャーナル | Sleep |
Abstract |
STUDY OBJECTIVES:Identifying the neurochemistry and neural circuitry of sleep regulation is critical for understanding sleep and various sleep disorders. Fruit flies display sleep-like behavior, sharing essential features with sleep of vertebrate. In the fruit fly's central brain, the mushroom body (MB) has been highlighted as a sleep center; however, its neurochemical nature remains unclear, and whether it promotes sleep or wake is still a topic of controversy. DESIGN:We used a video recording system to accurately monitor the locomotor activity and sleep status. Gene expression was temporally and regionally manipulated by heat induction and the Gal4/UAS system. MEASUREMENTS AND RESULTS:We found that expressing pertussis toxin (PTX) in the MB by c309-Gal4 to block Go activity led to unique sleep defects as dramatic sleep increase in daytime and fragmented sleep in nighttime. We narrowed down the c309-Gal4 expressing brain regions to the MB α/β core neurons that are responsible for the Go-mediated sleep effects. Using genetic tools of neurotransmitter-specific Gal80 and RNA interference approach to suppress acetylcholine signal, we demonstrated that these MB α/β core neurons were cholinergic and sleep-promoting neurons, supporting that Go mediates an inhibitory signal. Interestingly, we found that adjacent MB α/β neurons were also cholinergic but wake-promoting neurons, in which Go signal was also required. CONCLUSION:Our findings in fruit flies characterized a group of sleep-promoting neurons surrounded by a group of wake-promoting neurons. The two groups of neurons are both cholinergic and use Go inhibitory signal to regulate sleep. |
巻・号 | 36(12) |
ページ | 1809-21 |
公開日 | 2013-12-1 |
DOI | 10.5665/sleep.3206 |
PMID | 24293755 |
PMC | PMC3825430 |
MeSH | Animals Cholinergic Neurons / physiology* Circadian Rhythm / physiology Drosophila melanogaster / physiology Female GTP-Binding Protein alpha Subunits, Gi-Go / physiology* Humans Mushroom Bodies / physiology* Polymerase Chain Reaction Signal Transduction / physiology Sleep / physiology* |
IF | 4.805 |
引用数 | 23 |
WOS 分野 | CLINICAL NEUROLOGY NEUROSCIENCES |
リソース情報 | |
ショウジョウバエ | sca[109–68]-Gal4 (DGRC#108798) and NP lines |