論文 - 詳細
| RRC ID | 32547 |
|---|---|
| 著者 | Xiao G, Wan Z, Fan Q, Tang X, Zhou B. |
| タイトル | The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster. |
| ジャーナル | Elife |
| Abstract |
The intracellular iron transfer process is not well understood, and the identity of the iron transporter responsible for iron delivery to the secretory compartments remains elusive. In this study, we show Drosophila ZIP13 (Slc39a13), a presumed zinc importer, fulfills the iron effluxing role. Interfering with dZIP13 expression causes iron-rescuable iron absorption defect, simultaneous iron increase in the cytosol and decrease in the secretory compartments, failure of ferritin iron loading, and abnormal collagen secretion. dZIP13 expression in E. coli confers upon the host iron-dependent growth and iron resistance. Importantly, time-coursed transport assays using an iron isotope indicated a potent iron exporting activity of dZIP13. The identification of dZIP13 as an iron transporter suggests that the spondylocheiro dysplastic form of Ehlers-Danlos syndrome, in which hZIP13 is defective, is likely due to a failure of iron delivery to the secretory compartments. Our results also broaden our knowledge of the scope of defects from iron dyshomeostasis. |
| 巻・号 | 3 |
| ページ | e03191 |
| 公開日 | 2014-7-8 |
| DOI | 10.7554/eLife.03191 |
| PMID | 25006035 |
| PMC | PMC4130162 |
| MeSH | Amino Acid Sequence Animals Cation Transport Proteins / genetics Cation Transport Proteins / metabolism* Collagen / metabolism Drosophila Proteins / antagonists & inhibitors Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Ehlers-Danlos Syndrome / genetics Ehlers-Danlos Syndrome / metabolism* Ehlers-Danlos Syndrome / pathology Escherichia coli / genetics Escherichia coli / metabolism Ferritins / chemistry Ferritins / metabolism Gene Expression Humans Ion Transport Iron / metabolism Iron Radioisotopes Molecular Sequence Data Mutation Protein Isoforms / antagonists & inhibitors Protein Isoforms / genetics Protein Isoforms / metabolism RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Secretory Pathway / genetics* Sequence Alignment Sequence Homology, Amino Acid Transgenes |
| IF | 7.08 |
| 引用数 | 42 |
| WOS 分野 | BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | NP3084 (DGRC#113094) |