RRC ID 33166
著者 Satoh T, Ohba A, Liu Z, Inagaki T, Satoh AK.
タイトル dPob/EMC is essential for biosynthesis of rhodopsin and other multi-pass membrane proteins in Drosophila photoreceptors.
ジャーナル Elife
Abstract In eukaryotes, most integral membrane proteins are synthesized, integrated into the membrane, and folded properly in the endoplasmic reticulum (ER). We screened the mutants affecting rhabdomeric expression of rhodopsin 1 (Rh1) in the Drosophila photoreceptors and found that dPob/EMC3, EMC1, and EMC8/9, Drosophila homologs of subunits of ER membrane protein complex (EMC), are essential for stabilization of immature Rh1 in an earlier step than that at which another Rh1-specific chaperone (NinaA) acts. dPob/EMC3 localizes to the ER and associates with EMC1 and calnexin. Moreover, EMC is required for the stable expression of other multi-pass transmembrane proteins such as minor rhodopsins Rh3 and Rh4, transient receptor potential, and Na(+)K(+)-ATPase, but not for a secreted protein or type I single-pass transmembrane proteins. Furthermore, we found that dPob/EMC3 deficiency induces rhabdomere degeneration in a light-independent manner. These results collectively indicate that EMC is a key factor in the biogenesis of multi-pass transmembrane proteins, including Rh1, and its loss causes retinal degeneration.
巻・号 4
公開日 2015-2-26
DOI 10.7554/eLife.06306
PMID 25715730
PMC PMC4341237
MeSH Animals Drosophila / metabolism* Drosophila Proteins / metabolism* Membrane Proteins / metabolism* Photoreceptor Cells, Invertebrate / metabolism* Protein Transport Rhodopsin / biosynthesis*
IF 7.08
引用数 50
リソース情報
ショウジョウバエ P (RS3)[CB.0279.3] (DGRC#123106) y w ey-FLP CG6750[e02662] FRT40A/ CyO y[+] (DGRC#114504)