| RRC ID |
36173
|
| 著者 |
Rosas-Arellano A, Vásquez-Procopio J, Gambis A, Blowes LM, Steller H, Mollereau B, Missirlis F.
|
| タイトル |
Ferritin Assembly in Enterocytes of Drosophila melanogaster.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Ferritins are protein nanocages that accumulate inside their cavity thousands of oxidized iron atoms bound to oxygen and phosphates. Both characteristic types of eukaryotic ferritin subunits are present in secreted ferritins from insects, but here dimers between Ferritin 1 Heavy Chain Homolog (Fer1HCH) and Ferritin 2 Light Chain Homolog (Fer2LCH) are further stabilized by disulfide-bridge in the 24-subunit complex. We addressed ferritin assembly and iron loading in vivo using novel transgenic strains of Drosophila melanogaster. We concentrated on the intestine, where the ferritin induction process can be controlled experimentally by dietary iron manipulation. We showed that the expression pattern of Fer2LCH-Gal4 lines recapitulated iron-dependent endogenous expression of the ferritin subunits and used these lines to drive expression from UAS-mCherry-Fer2LCH transgenes. We found that the Gal4-mediated induction of mCherry-Fer2LCH subunits was too slow to effectively introduce them into newly formed ferritin complexes. Endogenous Fer2LCH and Fer1HCH assembled and stored excess dietary iron, instead. In contrast, when flies were genetically manipulated to co-express Fer2LCH and mCherry-Fer2LCH simultaneously, both subunits were incorporated with Fer1HCH in iron-loaded ferritin complexes. Our study provides fresh evidence that, in insects, ferritin assembly and iron loading in vivo are tightly regulated.
|
| 巻・号 |
17(2)
|
| ページ |
27
|
| 公開日 |
2016-2-5
|
| DOI |
10.3390/ijms17020027
|
| PII |
ijms17020027
|
| PMID |
26861293
|
| PMC |
PMC4783870
|
| MeSH |
Animals
Animals, Genetically Modified
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism*
Enterocytes / metabolism*
Ferritins / chemistry
Ferritins / genetics
Ferritins / metabolism*
Gastrointestinal Tract / metabolism
Gene Expression
Genes, Reporter
Genotype
Iron / metabolism
Larva
Models, Biological
Protein Subunits / genetics
Protein Subunits / metabolism
Protein Transport
Recombinant Fusion Proteins
|
| IF |
4.556
|
| 引用数 |
11
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CHEMISTRY, MULTIDISCIPLINARY
|
| リソース情報 |
| ショウジョウバエ |
DGRC#113517 DGRC#104255 |
