RRC ID 36235
著者 Sun X, Yang H, Sturgill D, Oliver B, Rabinow L, Samson ML.
タイトル Sxl-Dependent, tra/tra2-Independent Alternative Splicing of the Drosophila melanogaster X-Linked Gene found in neurons.
ジャーナル G3 (Bethesda)
Abstract Somatic sexual determination and behavior in Drosophila melanogaster are under the control of a genetic cascade initiated by Sex lethal (Sxl). In the female soma, SXL RNA-binding protein regulates the splicing of transformer (tra) transcripts into a female-specific form. The RNA-binding protein TRA and its cofactor TRA2 function in concert in females, whereas SXL, TRA, and TRA2 are thought to not function in males. To better understand sex-specific regulation of gene expression, we analyzed male and female head transcriptome datasets for expression levels and splicing, quantifying sex-biased gene expression via RNA-Seq and qPCR. Our data uncouple the effects of Sxl and tra/tra2 in females in the-sex-biased alternative splicing of head transcripts from the X-linked locus found in neurons (fne), encoding a pan-neuronal RNA-binding protein of the ELAV family. We show that FNE protein levels are downregulated by Sxl in female heads, also independently of tra/tra2. We argue that this regulation may have important sexually dimorphic consequences for the regulation of nervous system development or function.
巻・号 5(12)
ページ 2865-74
公開日 2015-10-28
DOI 10.1534/g3.115.023721
PII g3.115.023721
PMID 26511498
PMC PMC4683657
MeSH Alleles Alternative Splicing* Animals Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism* Female Gene Expression Profiling Gene Expression Regulation Genes, X-Linked* High-Throughput Nucleotide Sequencing Male Mutation Neurons / metabolism* Nuclear Proteins / genetics* RNA-Binding Proteins / metabolism* Ribonucleoproteins / genetics* Transcriptome
IF 2.781
引用数 6
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ DGRC#101894 DGRC#101842