RRC ID 47651
著者 Jun JW, Han G, Yun HM, Lee GJ, Hyun S.
タイトル Torso, a Drosophila receptor tyrosine kinase, plays a novel role in the larval fat body in regulating insulin signaling and body growth.
ジャーナル J Comp Physiol B
Abstract Torso is a receptor tyrosine kinase whose localized activation at the termini of the Drosophila embryo is mediated by its ligand, Trunk. Recent studies have unveiled a second function of Torso in the larval prothoracic gland (PG) as the receptor for the prothoracicotropic hormone, which triggers pupariation. As such, inhibition of Torso in the PG prolongs the larval growth period, thereby increasing the final pupa size. Here, we report that Torso also acts in the larval fat body, regulating body size in a manner opposite from that of Torso in PG. We confirmed the expression of torso mRNA in the larval fat body and its reduction by RNA interference (RNAi). Fat body-specific knockdown of torso, by either of the two independent RNAi transgenes, significantly decreased the final pupal size. We found that torso knockdown suppresses insulin/target of rapamycin (TOR) signaling in the fat body, as confirmed by repression of Akt and S6K. Notably, the decrease in insulin/TOR signaling and decrease of pupal size induced by the knockdown of torso were rescued by the expression of a constitutively active form of the insulin receptor or by the knockdown of FOXO. Our study revealed a novel role for Torso in the fat body with respect to regulation of insulin/TOR signaling and body size. This finding exemplifies the contrasting effects of the same gene expressed in two different organs on organismal physiology.
巻・号 186(6)
ページ 701-9
公開日 2016-8-1
DOI 10.1007/s00360-016-0992-2
PII 10.1007/s00360-016-0992-2
PMID 27126913
MeSH Animals Animals, Genetically Modified Body Size* Drosophila / genetics Drosophila / growth & development Drosophila / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila Proteins* / physiology Fat Body / metabolism* Insulin / metabolism* Larva / genetics Larva / growth & development Larva / metabolism Proto-Oncogene Proteins c-akt / metabolism Pupa / genetics Pupa / growth & development Pupa / metabolism RNA Interference RNA, Messenger / metabolism Receptor Protein-Tyrosine Kinases* / genetics Receptor Protein-Tyrosine Kinases* / metabolism Receptor Protein-Tyrosine Kinases* / physiology Ribosomal Protein S6 Kinases, 70-kDa / metabolism
IF 2.042
引用数 4
WOS 分野 PHYSIOLOGY ZOOLOGY
リソース情報
ショウジョウバエ DGRC#113244