RRC ID 57098
著者 Wilcockson SG, Ashe HL.
タイトル Drosophila Ovarian Germline Stem Cell Cytocensor Projections Dynamically Receive and Attenuate BMP Signaling.
ジャーナル Dev Cell
Abstract In the Drosophila ovarian germline, Bone Morphogenetic Protein (BMP) signals released by niche cells promote germline stem cell (GSC) maintenance. Although BMP signaling is known to repress expression of a key differentiation factor, it remains unclear whether BMP-responsive transcription also contributes positively to GSC identity. Here, we identify the GSC transcriptome using RNA sequencing (RNA-seq), including the BMP-induced transcriptional network. Based on these data, we provide evidence that GSCs form two types of cellular projections. Genetic manipulation and live ex vivo imaging reveal that both classes of projection allow GSCs to access a reservoir of Dpp held away from the GSC-niche interface. Moreover, microtubule-rich projections, termed "cytocensors", form downstream of BMP and have additional functionality, which is to attenuate BMP signaling. In this way, cytocensors allow dynamic modulation of signal transduction to facilitate differentiation following GSC division. This ability of cytocensors to attenuate the signaling response expands the repertoire of functions associated with signaling projections.
巻・号 50(3)
ページ 296-312.e5
公開日 2019-8-5
DOI 10.1016/j.devcel.2019.05.020
PII S1534-5807(19)30392-2
PMID 31178401
PMC PMC6688100
MeSH Animals Bone Morphogenetic Proteins / genetics Bone Morphogenetic Proteins / metabolism* Cell Differentiation Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster Female Gene Expression Regulation, Developmental Ovary / cytology* Signal Transduction* Stem Cell Niche* Stem Cells / cytology Stem Cells / metabolism*
IF 9.19
引用数 3
リソース情報
ショウジョウバエ DGRC#109171