RRC ID |
57299
|
著者 |
Martin JL, Sanders EN, Moreno-Roman P, Jaramillo Koyama LA, Balachandra S, Du X, O'Brien LE.
|
タイトル |
Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss.
|
ジャーナル |
Elife
|
Abstract |
Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult Drosophila midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the midgut to be imaged while intact and physiologically functioning. This approach prolongs imaging sessions to 12-16 hr and yields movies that document cell and tissue dynamics at vivid spatiotemporal resolution. By applying a pipeline for movie processing and analysis, we uncover new and intriguing cell behaviors: that mitotic stem cells dynamically re-orient, that daughter cells use slow kinetics of Notch activation to reach a fate-specifying threshold, and that enterocytes extrude via ratcheted constriction of a junctional ring. By enabling real-time study of midgut phenomena that were previously inaccessible, our platform opens a new realm for dynamic understanding of adult organ renewal.
|
巻・号 |
7
|
公開日 |
2018-11-14
|
DOI |
10.7554/eLife.36248
|
PII |
36248
|
PMID |
30427308
|
PMC |
PMC6277200
|
MeSH |
Aging / physiology*
Animals
Cell Communication
Cell Differentiation*
Cell Division*
Cell Lineage
Cell Nucleus / metabolism
Cell Survival
Cell Tracking
Digestive System / cytology*
Drosophila melanogaster / cytology*
Drosophila melanogaster / physiology*
Enterocytes / cytology
Imaging, Three-Dimensional
Kinetics
Mitosis
Receptors, Notch / metabolism
Single-Cell Analysis
Stem Cells / cytology
|
IF |
7.551
|
引用数 |
8
|
リソース情報 |
ショウジョウバエ |
DGRC#112304 |