RRC ID 57341
著者 Sharma SK, Ghosh S, Geetha AR, Mandal S, Mandal L.
タイトル Cell Adhesion-Mediated Actomyosin Assembly Regulates the Activity of Cubitus Interruptus for Hematopoietic Progenitor Maintenance in Drosophila.
ジャーナル Genetics
Abstract The actomyosin network is involved in crucial cellular processes including morphogenesis, cell adhesion, apoptosis, proliferation, differentiation, and collective cell migration in Drosophila, Caenorhabditiselegans, and mammals. Here, we demonstrate that Drosophila larval blood stem-like progenitors require actomyosin activity for their maintenance. Genetic loss of the actomyosin network from progenitors caused a decline in their number. Likewise, the progenitor population increased upon sustained actomyosin activation via phosphorylation by Rho-associated kinase. We show that actomyosin positively regulates larval blood progenitors by controlling the maintenance factor Cubitus interruptus (Ci). Overexpression of the maintenance signal via a constitutively activated construct (ci.HA) failed to sustain Ci-155 in the absence of actomyosin components like Zipper (zip) and Squash (sqh), thus favoring protein kinase A (PKA)-independent regulation of Ci activity. Furthermore, we demonstrate that a change in cortical actomyosin assembly mediated by DE-cadherin modulates Ci activity, thereby determining progenitor status. Thus, loss of cell adhesion and downstream actomyosin activity results in desensitization of the progenitors to Hh signaling, leading to their differentiation. Our data reveal how cell adhesion and the actomyosin network cooperate to influence patterning, morphogenesis, and maintenance of the hematopoietic stem-like progenitor pool in the developing Drosophila hematopoietic organ.
巻・号 212(4)
ページ 1279-1300
公開日 2019-8-1
DOI 10.1534/genetics.119.302209
PII genetics.119.302209
PMID 31138608
PMC PMC6707476
MeSH Actomyosin / metabolism* Animals Cadherins / metabolism Cyclic AMP-Dependent Protein Kinases / metabolism DNA-Binding Proteins / metabolism* Drosophila Proteins / metabolism* Drosophila melanogaster Hematopoiesis Hematopoietic Stem Cells / metabolism* Membrane Proteins / metabolism Myosin Heavy Chains / metabolism Myosin Type II / metabolism Protein Multimerization Transcription Factors / metabolism* rho-Associated Kinases / metabolism
IF 3.564
引用数 3
リソース情報
ショウジョウバエ DGRC#105442 DGRC#115452