RRC ID 57349
著者 Alyagor I, Berkun V, Keren-Shaul H, Marmor-Kollet N, David E, Mayseless O, Issman-Zecharya N, Amit I, Schuldiner O.
タイトル Combining Developmental and Perturbation-Seq Uncovers Transcriptional Modules Orchestrating Neuronal Remodeling.
ジャーナル Dev Cell
Abstract Developmental neuronal remodeling is an evolutionarily conserved mechanism required for precise wiring of nervous systems. Despite its fundamental role in neurodevelopment and proposed contribution to various neuropsychiatric disorders, the underlying mechanisms are largely unknown. Here, we uncover the fine temporal transcriptional landscape of Drosophila mushroom body γ neurons undergoing stereotypical remodeling. Our data reveal rapid and dramatic changes in the transcriptional landscape during development. Focusing on DNA binding proteins, we identify eleven that are required for remodeling. Furthermore, we sequence developing γ neurons perturbed for three key transcription factors required for pruning. We describe a hierarchical network featuring positive and negative feedback loops. Superimposing the perturbation-seq on the developmental expression atlas highlights a framework of transcriptional modules that together drive remodeling. Overall, this study provides a broad and detailed molecular insight into the complex regulatory dynamics of developmental remodeling and thus offers a pipeline to dissect developmental processes via RNA profiling.
巻・号 47(1)
ページ 38-52.e6
公開日 2018-10-8
DOI 10.1016/j.devcel.2018.09.013
PII S1534-5807(18)30742-1
PMID 30300589
PMC PMC6179959
MeSH Animals Axons / metabolism DNA-Binding Proteins / metabolism Drosophila Proteins / metabolism Drosophila melanogaster / metabolism Gene Expression Regulation, Developmental / genetics Metamorphosis, Biological / physiology Mushroom Bodies / embryology* Mushroom Bodies / metabolism Neuronal Plasticity / genetics* Neuronal Plasticity / physiology* Neurons / metabolism Sequence Analysis, RNA Transcription Factors / metabolism Transcriptome
IF 9.19
引用数 9
リソース情報
ショウジョウバエ DGRC#104360