論文 - 詳細
| RRC ID | 59130 |
|---|---|
| 著者 | Sênos Demarco R, Uyemura BS, Jones DL. |
| タイトル | EGFR Signaling Stimulates Autophagy to Regulate Stem Cell Maintenance and Lipid Homeostasis in the Drosophila Testis. |
| ジャーナル | Cell Rep |
| Abstract |
Although typically upregulated upon cellular stress, autophagy can also be utilized under homeostatic conditions as a quality control mechanism or in response to developmental cues. Here, we report that autophagy is required for the maintenance of somatic cyst stem cells (CySCs) in the Drosophila testis. Disruption of autophagy in CySCs and early cyst cells (CCs) by the depletion of autophagy-related (Atg) genes reduced early CC numbers and affected CC function, resembling decreased epidermal growth factor receptor (EGFR) signaling. Indeed, our data indicate that EGFR acts to stimulate autophagy to preserve early CC function, whereas target of rapamycin (TOR) negatively regulates autophagy in the differentiating CCs. Finally, we show that the EGFR-mediated stimulation of autophagy regulates lipid levels in CySCs and CCs. These results demonstrate a key role for autophagy in regulating somatic stem cell behavior and tissue homeostasis by integrating cues from both the EGFR and TOR signaling pathways to control lipid metabolism. |
| 巻・号 | 30(4) |
| ページ | 1101-1116.e5 |
| 公開日 | 2020-1-28 |
| DOI | 10.1016/j.celrep.2019.12.086 |
| PII | S2211-1247(19)31751-6 |
| PMID | 31995752 |
| PMC | PMC7357864 |
| MeSH | Animals Animals, Genetically Modified Autophagosomes / metabolism Autophagy / genetics* Cell Differentiation / genetics Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* ErbB Receptors / genetics ErbB Receptors / metabolism* Gene Knockdown Techniques Germ Cells / growth & development Germ Cells / metabolism* Homeostasis Lipid Metabolism / genetics* MAP Kinase Signaling System / genetics Male RNA Interference Receptors, Invertebrate Peptide / genetics Receptors, Invertebrate Peptide / metabolism* Stem Cells / metabolism* TOR Serine-Threonine Kinases / metabolism Testis / cytology Testis / metabolism Transcription Factor AP-1 / genetics Transcription Factor AP-1 / metabolism |
| IF | 8.109 |
| 引用数 | 3 |
| リソース情報 | |
| ショウジョウバエ | DGRC#104055 |