| RRC ID |
70756
|
| 著者 |
Resnik-Docampo M, Cunningham KM, Ruvalcaba SM, Choi C, Sauer V, Jones DL.
|
| タイトル |
Neuroglian regulates Drosophila intestinal stem cell proliferation through enhanced signaling via the epidermal growth factor receptor.
|
| ジャーナル |
Stem Cell Reports
|
| Abstract |
The Drosophila intestine is an excellent system for elucidating mechanisms regulating stem cell behavior. Here we show that the septate junction (SJ) protein Neuroglian (Nrg) is expressed in intestinal stem cells (ISCs) and enteroblasts (EBs) within the fly intestine. SJs are not present between ISCs and EBs, suggesting Nrg plays a different role in this tissue. We reveal that Nrg is required for ISC proliferation in young flies, and depletion of Nrg from ISCs and EBs suppresses increased ISC proliferation in aged flies. Conversely, overexpression of Nrg in ISC and EBs promotes ISC proliferation, leading to an increase in cells expressing ISC/EB markers; in addition, we observe an increase in epidermal growth factor receptor (Egfr) activation. Genetic epistasis experiments reveal that Nrg acts upstream of Egfr to regulate ISC proliferation. As Nrg function is highly conserved in mammalian systems, our work characterizing the role of Nrg in the intestine has implications for the treatment of intestinal disorders that arise due to altered ISC behavior.
|
| 巻・号 |
16(6)
|
| ページ |
1584-1597
|
| 公開日 |
2021-6-8
|
| DOI |
10.1016/j.stemcr.2021.04.006
|
| PII |
S2213-6711(21)00201-0
|
| PMID |
33961791
|
| PMC |
PMC8190597
|
| MeSH |
Aging / metabolism
Animals
Cell Adhesion Molecules, Neuronal / genetics
Cell Adhesion Molecules, Neuronal / metabolism*
Cell Proliferation
Drosophila / metabolism*
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
ErbB Receptors / metabolism*
Female
Gene Expression Regulation, Developmental
Intestines / cytology
Intestines / metabolism*
Signal Transduction
Stem Cells / metabolism*
|
| IF |
6.032
|
| リソース情報 |
| ショウジョウバエ |
DGRC#111923 |
