RRC ID 70784
著者 Tatapudy S, Peralta J, Nystul T.
タイトル Distinct roles of Bendless in regulating FSC niche competition and daughter cell differentiation.
ジャーナル Development
Abstract A major goal in the study of adult stem cells is to understand how cell fates are specified at the proper time and place to facilitate tissue homeostasis. Here, we found that an E2 ubiquitin ligase, Bendless (Ben), has multiple roles in the Drosophila ovarian epithelial follicle stem cell (FSC) lineage. First, Ben is part of the JNK signaling pathway, and we found that it, as well as other JNK pathway genes, are essential for differentiation of FSC daughter cells. Our data suggest that JNK signaling promotes differentiation by suppressing the activation of the EGFR effector, ERK. Also, we found that loss of ben, but not the JNK kinase hemipterous, resulted in an upregulation of hedgehog signaling, increased proliferation and increased niche competition. Lastly, we demonstrate that the hypercompetition phenotype caused by loss of ben is suppressed by decreasing the rate of proliferation or knockdown of the hedgehog pathway effector, Smoothened (Smo). Taken together, our findings reveal a new layer of regulation in which a single gene influences cell signaling at multiple stages of differentiation in the early FSC lineage.
巻・号 148(22)
公開日 2021-11-15
DOI 10.1242/dev.199630
PII 273497
PMID 35020878
PMC PMC8645206
MeSH Animals Cell Differentiation / genetics Cell Lineage / genetics Cell Proliferation / genetics Drosophila Proteins / genetics* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Epithelial Cells / cytology ErbB Receptors / genetics* Female Hedgehog Proteins / genetics* MAP Kinase Signaling System / genetics Ovarian Follicle / growth & development* Ovarian Follicle / metabolism Receptors, Invertebrate Peptide / genetics* Smoothened Receptor / genetics* Stem Cell Niche / genetics Stem Cells / cytology Ubiquitin-Conjugating Enzymes / genetics*
IF 5.611
リソース情報
ショウジョウバエ DGRC#111740