RRC ID 48516
著者 Ojima-Kato T, Yamamoto N, Takahashi H, Tamura H.
タイトル Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Can Precisely Discriminate the Lineages of Listeria monocytogenes and Species of Listeria.
ジャーナル PLoS One
Abstract The genetic lineages of Listeria monocytogenes and other species of the genus Listeria are correlated with pathogenesis in humans. Although matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has become a prevailing tool for rapid and reliable microbial identification, the precise discrimination of Listeria species and lineages remains a crucial issue in clinical settings and for food safety. In this study, we constructed an accurate and reliable MS database to discriminate the lineages of L. monocytogenes and the species of Listeria (L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii, L. grayi, and L. rocourtiae) based on the S10-spc-alpha operon gene encoded ribosomal protein mass spectrum (S10-GERMS) proteotyping method, which relies on both genetic information (genomics) and observed MS peaks in MALDI-TOF MS (proteomics). The specific set of eight biomarkers (ribosomal proteins L24, L6, L18, L15, S11, S9, L31 type B, and S16) yielded characteristic MS patterns for the lineages of L. monocytogenes and the different species of Listeria, and led to the construction of a MS database that was successful in discriminating between these organisms in MALDI-TOF MS fingerprinting analysis followed by advanced proteotyping software Strain Solution analysis. We also confirmed the constructed database on the proteotyping software Strain Solution by using 23 Listeria strains collected from natural sources.
巻・号 11(7)
ページ e0159730
公開日 2016-1-1
DOI 10.1371/journal.pone.0159730
PII PONE-D-16-14587
PMID 27442502
PMC PMC4956195
MeSH Computational Biology / methods DNA Fingerprinting / methods Databases, Genetic Listeria / classification* Listeria / genetics Listeria monocytogenes / classification* Listeria monocytogenes / genetics Software Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods
IF 2.74
引用数 15
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 2873 JCM 7671 JCM 7672 JCM 7673 JCM 7674 JCM 7675 JCM 7676 JCM 7677 JCM 7678 JCM 7679 JCM 7680 JCM 7681 JCM 7682 JCM 7683