RRC ID 49997
著者 Ishinishi R, Matsuura H, Tanaka S, Nozawa S, Tanada K, Kawashita N, Fujiyama K, Miyasaka H, Hirata K.
タイトル Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga.
ジャーナル Gene
Abstract The genetic basis of stress resistance in extremophilic microalgae is not well studied. In this study, a gene of unknown function, the cluster58 or CL58 gene, was identified from the halotolerant green alga Chlamydomonas W80 and characterized. The CL58 gene encodes a protein containing a domain of unknown function, the CHRD domain, and a putative secretory signaling sequence at its N-terminus. The levels of CL58 mRNA increased in response to high copper levels and low temperatures. When the CL58 gene was heterologously expressed as a fusion gene with the NanoLuc luciferase gene in Chlamydomonas reinhardtii, a majority of the NanoLuc activity was detected in the culture medium compared with that in the intracellular fraction. A mutagenic analysis revealed that the putative secretory signaling sequence was sufficient for the secretion of the CL58-NanoLuc fusion protein. In addition, we expressed the protein encoded by the CL58 gene in Escherichia coli; the recombinant, soluble protein was then purified. In summary, we identified a novel gene from C. W80 that appears to encode a stress-responsive, CHRD domain-containing secreted protein.
巻・号 640
ページ 14-20
公開日 2018-1-15
DOI 10.1016/j.gene.2017.10.012
PII S0378-1119(17)30825-9
PMID 29017964
MeSH Amino Acid Sequence Animals Base Sequence Chlamydomonas reinhardtii / genetics* Chlamydomonas reinhardtii / growth & development Chlamydomonas reinhardtii / metabolism Cold Temperature Copper / toxicity DNA, Algal / genetics* Glycoproteins / chemistry* Intercellular Signaling Peptides and Proteins / chemistry* Plant Proteins / genetics* Plant Proteins / metabolism Protein Domains Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Homology Stress, Physiological / genetics* Transgenes / genetics*
IF 2.984
引用数 0
リソース情報
藻類 NIES-2235