論文 - 詳細
| RRC ID | 30794 |
|---|---|
| 著者 | Hofstetter SS, Dudnik A, Widmer H, Dudler R. |
| タイトル | Arabidopsis YELLOW STRIPE-LIKE7 (YSL7) and YSL8 transporters mediate uptake of Pseudomonas virulence factor syringolin A into plant cells. |
| ジャーナル | Mol Plant Microbe Interact |
| Abstract |
Syringolin A (SylA), a virulence factor secreted by certain strains of the plant pathogen Pseudomonas syringae pv. syringae, is an irreversible proteasome inhibitor imported by plant cells by an unknown transport process. Here, we report that functional expression in yeast of all 17 members of the Arabidopsis oligopeptide transporter family revealed that OLIGOPEPTIDE TRANSPORTER1 (OPT1), OPT2, YELLOW STRIPE-LIKE3 (YSL3), YSL7, and YSL8 rendered yeast cells sensitive to growth inhibition by SylA to different degrees, strongly indicating that these proteins mediated SylA uptake into yeast cells. The greatest SylA sensitivity was conferred by YSL7 and YSL8 expression. An Arabidopsis ysl7 mutant exhibited strongly reduced SylA sensitivity in a root growth inhibition assay and in leaves of ysl7 and ysl8 mutants, SylA-mediated quenching of salicylic-acid-triggered PATHOGENESIS-RELATED GENE1 transcript accumulation was greatly reduced compared with the wild type. These results suggest that YSL7 and YSL8 are major SylA uptake transporters in Arabidopsis. Expression of a YSL homolog of bean, the host of the SylA-producing P. syringae pv. syringae B728a, in yeast also conferred strong SylA sensitivity. Thus, YSL transporters, which are thought to be involved in metal homeostasis, have been hijacked by bacterial pathogens for SylA uptake into host cells. |
| 巻・号 | 26(11) |
| ページ | 1302-11 |
| 公開日 | 2013-11-1 |
| DOI | 10.1094/MPMI-06-13-0163-R |
| PMID | 23945001 |
| MeSH | Arabidopsis / drug effects Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis / microbiology Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Biological Transport Gene Expression Genes, Reporter Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism* Mutation Oligopeptides / metabolism Organ Specificity Peptides, Cyclic / metabolism* Peptides, Cyclic / pharmacology Phylogeny Plant Diseases / microbiology* Plant Leaves / drug effects Plant Leaves / genetics Plant Leaves / metabolism Plant Leaves / microbiology Plant Roots / drug effects Plant Roots / genetics Plant Roots / metabolism Plant Roots / microbiology Pseudomonas syringae / metabolism* Recombinant Fusion Proteins Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / growth & development Saccharomyces cerevisiae / metabolism Seedlings / drug effects Seedlings / genetics Seedlings / metabolism Seedlings / microbiology Virulence Factors / metabolism |
| IF | 3.696 |
| 引用数 | 8 |
| WOS 分野 | PLANT SCIENCES BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda01488 pda02028 pda02432 pda07074 pda09151 |