RRC ID 41198
著者 Niu L, Xiong M, Tang T, Song L, Hu X, Zhao M, Zhang K.
タイトル Aeromicrobium camelliae sp. nov., isolated from Pu'er tea.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel Gram-reaction-positive, aerobic and non-spore-forming rod-shaped bacterial strain, YS17T, was isolated from ripened Pu'er tea. Growth of the strain was observed at 15-50 °C (optimum 30-37 °C) and at pH 5.5-10.5 (optimum 6.0-9.5). Phylogenetic analysis of 16S rRNA gene sequences indicated that the strain represented a member of the genus Aeromicrobium. The strains most closely related to YS17T were Aeromicrobium erythreum DSM 8599T, Aeromicrobium alkaliterrae JCM 13518T and Aeromicrobium ginsengisoli JCM 14732T, with 16S rRNA gene sequence similarities of 96.8, 96.8 and 96.7 %, respectively. DNA-DNA hybridization of YS17T with the type strains of the most closely related species, A. erythreum DSM 8599T, A. alkaliterrae JCM 13518T and A. ginsengisoli JCM 14732T, yielded reassociation values of 10.9, 16.8 and 10.9 %, respectively. The diagnostic diamino acid of the cell wall peptidoglycan was ll-diaminopimelic acid. The predominant menaquinones were menaquinone MK-9(H4) (76 %) and MK-8(H4) (17 %). The major fatty acids were C16 : 0, 10-methyl C18 : 0 and C18 : 1ω9c. The DNA G+C content of YS17T was 66 mol%. YS17T could be differentiated from recognized species of the genus Aeromicrobium on the basis of phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. On the basis of evidence from the polyphasic analyses performed as part of this study a novel species, Aeromicrobium camelliae sp. nov., is proposed, with strain YS17T ( = CGMCC 1.12942T = JCM 30952T) as the type strain.
巻・号 65(12)
ページ 4369-4373
公開日 2015-12-1
DOI 10.1099/ijsem.0.000583
PMID 26354190
MeSH Actinomycetales / classification* Actinomycetales / genetics Actinomycetales / isolation & purification Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry Molecular Sequence Data Nucleic Acid Hybridization Peptidoglycan / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Tea / microbiology* Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.415
引用数 8
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 13518 JCM 14732  JCM 30952