RRC ID 41209
著者 Liu Y, Zhai L, Wang R, Zhao R, Zhang X, Chen C, Cao Y, Cao Y, Xu T, Ge Y, Zhao J, Cheng C.
タイトル Paenibacillus zeae sp. nov., isolated from maize (Zea mays L.) seeds.
ジャーナル Int J Syst Evol Microbiol
Abstract Four Gram-stain-positive bacterial strains, designated 6R2T, 6R18, 3T2 and 3T10, isolated from seeds of hybrid maize (Zea mays L., Jingke 968) were investigated using a polyphasic taxonomic approach. Cells were aerobic, motile, spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolates may represent a novel species of the genus Paenibacillus, the four closest neighbours being Paenibacillus lautus NRRL NRS-666T (97.1 % similarity), Paenibacillus glucanolyticus DSM 5162T (97.0 %), Paenibacillus lactis MB 1871T (97.0 %) and Paenibacillus chibensis JCM 9905T (96.8 %). The DNA G+C content of strain 6R2T was 51.8 mol%. Its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C15 : 0 and iso-C14 : 0. Strains 6R2T, 6R18, 3T2 and 3T10 were clearly distinguished from the above type strains using phylogenetic analysis, DNA-DNA hybridization, and a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strains 6R2T, 6R18, 3T2 and 3T10 represent a novel species of the genus Paenibacillus, for which the name Paenibacillus zeae sp. nov. is proposed. The type strain is 6R2T ( = KCTC 33674T = CICC 23860T).
巻・号 65(12)
ページ 4533-4538
公開日 2015-12-1
DOI 10.1099/ijsem.0.000608
PMID 26373452
MeSH Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Endophytes / classification Endophytes / genetics Endophytes / isolation & purification Fatty Acids / chemistry Molecular Sequence Data Nucleic Acid Hybridization Paenibacillus / classification* Paenibacillus / genetics Paenibacillus / isolation & purification Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Seeds / microbiology* Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry Zea mays / microbiology*
IF 2.415
引用数 9
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 9905