RRC ID 51440
著者 Li Y, Zhao D, Horie T, Chen G, Bao H, Chen S, Liu W, Horie R, Liang T, Dong B, Feng Q, Tao Q, Liu X.
タイトル Conserved gene regulatory module specifies lateral neural borders across bilaterians.
ジャーナル Proc Natl Acad Sci U S A
Abstract The lateral neural plate border (NPB), the neural part of the vertebrate neural border, is composed of central nervous system (CNS) progenitors and peripheral nervous system (PNS) progenitors. In invertebrates, PNS progenitors are also juxtaposed to the lateral boundary of the CNS. Whether there are conserved molecular mechanisms determining vertebrate and invertebrate lateral neural borders remains unclear. Using single-cell-resolution gene-expression profiling and genetic analysis, we present evidence that orthologs of the NPB specification module specify the invertebrate lateral neural border, which is composed of CNS and PNS progenitors. First, like in vertebrates, the conserved neuroectoderm lateral border specifier Msx/vab-15 specifies lateral neuroblasts in Caenorhabditis elegans Second, orthologs of the vertebrate NPB specification module (Msx/vab-15, Pax3/7/pax-3, and Zic/ref-2) are significantly enriched in worm lateral neuroblasts. In addition, like in other bilaterians, the expression domain of Msx/vab-15 is more lateral than those of Pax3/7/pax-3 and Zic/ref-2 in C. elegans Third, we show that Msx/vab-15 regulates the development of mechanosensory neurons derived from lateral neural progenitors in multiple invertebrate species, including C. elegans, Drosophila melanogaster, and Ciona intestinalis We also identify a novel lateral neural border specifier, ZNF703/tlp-1, which functions synergistically with Msx/vab-15 in both C. elegans and Xenopus laevis These data suggest a common origin of the molecular mechanism specifying lateral neural borders across bilaterians.
巻・号 114(31)
ページ E6352-E6360
公開日 2017-8-1
DOI 10.1073/pnas.1704194114
PII 1704194114
PMID 28716930
PMC PMC5547623
MeSH Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / metabolism Carrier Proteins / metabolism Ciona intestinalis / embryology* Drosophila melanogaster / embryology* Gene Expression Regulation, Developmental / physiology* MSX1 Transcription Factor / metabolism Neural Crest / embryology* Neural Plate / embryology* Neural Stem Cells / metabolism* Paired Box Transcription Factors / metabolism Peripheral Nervous System / cytology Peripheral Nervous System / embryology Single-Cell Analysis Xenopus laevis / embryology*
IF 9.412
引用数 16
リソース情報
線虫 tm1446 tm1688 tm1803 tm260