RRC ID 48579
著者 Suorsa M, Rossi F, Tadini L, Labs M, Colombo M, Jahns P, Kater MM, Leister D, Finazzi G, Aro EM, Barbato R, Pesaresi P.
タイトル PGR5-PGRL1-Dependent Cyclic Electron Transport Modulates Linear Electron Transport Rate in Arabidopsis thaliana.
ジャーナル Mol Plant
Abstract Plants need tight regulation of photosynthetic electron transport for survival and growth under environmental and metabolic conditions. For this purpose, the linear electron transport (LET) pathway is supplemented by a number of alternative electron transfer pathways and valves. In Arabidopsis, cyclic electron transport (CET) around photosystem I (PSI), which recycles electrons from ferrodoxin to plastoquinone, is the most investigated alternative route. However, the interdependence of LET and CET and the relative importance of CET remain unclear, largely due to the difficulties in precise assessment of the contribution of CET in the presence of LET, which dominates electron flow under physiological conditions. We therefore generated Arabidopsis mutants with a minimal water-splitting activity, and thus a low rate of LET, by combining knockout mutations in PsbO1, PsbP2, PsbQ1, PsbQ2, and PsbR loci. The resulting Δ5 mutant is viable, although mature leaves contain only ∼ 20% of wild-type naturally less abundant PsbO2 protein. Δ5 plants compensate for the reduction in LET by increasing the rate of CET, and inducing a strong non-photochemical quenching (NPQ) response during dark-to-light transitions. To identify the molecular origin of such a high-capacity CET, we constructed three sextuple mutants lacking the qE component of NPQ (Δ5 npq4-1), NDH-mediated CET (Δ5 crr4-3), or PGR5-PGRL1-mediated CET (Δ5 pgr5). Their analysis revealed that PGR5-PGRL1-mediated CET plays a major role in ΔpH formation and induction of NPQ in C3 plants. Moreover, while pgr5 dies at the seedling stage under fluctuating light conditions, Δ5 pgr5 plants are able to survive, which underlines the importance of PGR5 in modulating the intersystem electron transfer.
巻・号 9(2)
ページ 271-288
公開日 2016-2-1
DOI 10.1016/j.molp.2015.12.001
PII S1674-2052(15)00454-2
PMID 26687812
MeSH Arabidopsis / chemistry Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis / radiation effects Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Electron Transport Kinetics Light Membrane Proteins / genetics Membrane Proteins / metabolism* Photosynthesis / radiation effects Photosynthetic Reaction Center Complex Proteins / genetics Photosynthetic Reaction Center Complex Proteins / metabolism* Photosystem I Protein Complex / genetics Photosystem I Protein Complex / metabolism
IF 12.084
引用数 47
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pst13573