RRC ID 50293
著者 Suekawa M, Fujikawa Y, Esaka M.
タイトル Two G-box-like elements essential to high gene expression of SlAKR4B in tomato leaves.
ジャーナル Biosci Biotechnol Biochem
Abstract Aldo-keto reductases (AKRs) play important roles in aldehyde detoxification as well as primary and secondary metabolism in plants. We previously reported inducible expression of a Solanum lycopersicum AKR4B (SlAKR4B) in tomato leaves treated with salicylic acid and jasmonic acid, and high promoter activity of SlAKR4B in tomato leaf protoplasts. In this study, we investigated the expression response of SlAKR4B in the tomato leaves with infiltration treatment and the cis-element(s) involved in high promoter activity. Gene expression analysis in tomato leaf protoplasts and buffer-infiltrated tomato leaves suggested that cell damage caused the increased expression of SlAKR4B. Promoter activity of SlAKR4B was significantly reduced by mutation of two G-box like elements. It is suggested that the two G-box like elements are responsible for the high promoter activity.
巻・号 82(3)
ページ 425-432
公開日 2018-3-1
DOI 10.1080/09168451.2018.1429887
PMID 29384041
MeSH Aldo-Keto Reductases / genetics* Gene Expression Regulation, Plant* Plant Leaves / genetics* Promoter Regions, Genetic / genetics* Protoplasts / metabolism Solanum lycopersicum / cytology Solanum lycopersicum / enzymology* Solanum lycopersicum / genetics* Transcription, Genetic
IF 1.516
引用数 0
リソース情報
トマト TOMJPF00001