RRC ID 50664
著者 Jin L, Ko SR, Cui Y, Lee CS, Oh HM, Ahn CY, Lee HG.
タイトル Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor.
ジャーナル Antonie Van Leeuwenhoek
Abstract A polyphasic taxonomic study was carried out on strain EBR-8-1T isolated from a biofilm reactor in Korea. The cells of the strain were Gram-stain negative, non-spore-forming, non-motile, and short rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain with Betaproteobacteria, which showed high pairwise sequence similarities with Pusillimonas noertemannii BN9T (99.1 %), Pusillimonas soli MJ07T (97.3 %), Pusillimonas ginsengisoli DCY25T (97.2 %), and Pusillimonas harenae B201T (96.8 %). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage within the genus Pusillimonas. The major fatty acids were identified as C16:0, C17:0 cyclo and C19:0 cyclo ω8c. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The respiratory quinone was identified as Q-8 and the genomic DNA G+C content was determined to be 63.3 mol%. On the basis of polyphasic evidence, it is proposed that strain EBR-8-1T should be placed in a new species, Pusillimonas caeni sp. nov. The type stain is EBR-8-1T (=KCTC 42353T = JCM 30463T).
巻・号 110(1)
ページ 125-132
公開日 2017-1-1
DOI 10.1007/s10482-016-0782-6
PII 10.1007/s10482-016-0782-6
PMID 27744638
MeSH Alcaligenaceae / classification Alcaligenaceae / genetics Alcaligenaceae / isolation & purification* Alcaligenaceae / metabolism Bacterial Typing Techniques Base Composition Biofilms* DNA, Bacterial / genetics Fatty Acids / chemistry Fatty Acids / metabolism Phylogeny RNA, Ribosomal, 16S / genetics Republic of Korea Sewage / microbiology*
IF 2.674
引用数 9
リソース情報
一般微生物 JCM 30463