RRC ID 50756
著者 Paek J, Shin JH, Shin Y, Park IS, Kim H, Kook JK, Kang SS, Kim DS, Park KH, Chang YH.
タイトル Vibrio injenensis sp. nov., isolated from human clinical specimens.
ジャーナル Antonie Van Leeuwenhoek
Abstract Vibrio species are well known as motile, mostly oxidase-positive, facultative anaerobic Gram-negative bacteria. They are abundant in aquatic environments and are a common cause of human infections including diarrhea, soft tissue diseases, and bacteremia. Here, two Gram-negative bacteria, designated M12-1144T and M12-1181, were isolated from human clinical specimens and identified using a polyphasic taxonomic approach. Phylogenetic study based on 16S rRNA gene sequence analysis revealed that the isolates belong to the genus Vibrio, and are closely related to Vibrio metschnikovii KCTC 32284T (98.3%) and Vibrio cincinnatiensis KCTC 2733T (97.8%). The major fatty acids were summed feature 3 (C16:1 ω7c/C16:1 ω6c, 38.0%), C16:0 (23.0%), and summed feature 8 (C18:1 ω7c or C18:1 ω6c, 19.3%) and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The G + C content of the genomic DNA was determined to be 44.1 mol%. DNA-DNA relatedness between the two newly isolated strains and V. metschnikovii KCTC 32284T and V. cincinnatiensis KCTC 2733T was between 42.6 to 47.5%. The similarities of genome-to-genome distance between M12-1144T and related species ranged from 18.4-54.8%. Based on these results, a new species of the genus Vibrio, Vibrio injenensis is proposed. The type strain is M12-1144 T(=KCTC 32233T =JCM 30011T).
巻・号 110(1)
ページ 145-152
公開日 2017-1-1
DOI 10.1007/s10482-016-0810-6
PII 10.1007/s10482-016-0810-6
PMID 28012139
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics DNA, Ribosomal / genetics Fatty Acids / chemistry Fatty Acids / metabolism Humans Phylogeny RNA, Ribosomal, 16S / genetics Vibrio / classification Vibrio / isolation & purification* Vibrio / metabolism Vibrio Infections / microbiology*
IF 2.674
引用数 8
リソース情報
一般微生物 JCM 30011