RRC ID 43339
著者 Hosaka H, Nagata A, Yoshida T, Shibata T, Nagao T, Tanaka T, Saito Y, Tatsuno I.
タイトル Pancreatic polypeptide is secreted from and controls differentiation through its specific receptors in osteoblastic MC3T3-E1 cells.
ジャーナル Peptides
Abstract Although the neuropeptide Y (NPY) family has been demonstrated to control bone metabolism, the role of pancreatic polypeptide (PP), which has structural homology with NPY and peptide YY (PYY) to share the NPY family receptors, in peripheral bone tissues has remained unknown. In the present study, we studied the regulatory roles of PP and its Y receptors using MC3T3-E1 cells, a murine transformed osteoblastic cell line, as a model for osteoblastic differentiation. We found that (1) PP mRNA was detected and increased during cell-contact-induced differentiation in MC3T3-E1 cells; (2) the immunoreactivity of PP was detected by radioimmunoassay and increased in culture medium during differentiation; (3) all the types of NPY family receptor mRNAs (Y1, Y2, Y4, Y5, and y6) were found to increase during differentiation; (4) PP stimulated differentiation in MC3T3-E1 cells in terms of ALP mRNA and BMP-2 mRNA. These findings suggested that MC3T3-E1 cells produce and secrete PP, which may in turn stimulate the differentiation of MC3T3-E1 through its specific receptors in an autocrine manner.
巻・号 29(8)
ページ 1390-5
公開日 2008-8-1
DOI 10.1016/j.peptides.2008.03.015
PII S0196-9781(08)00134-4
PMID 18440094
MeSH Animals Cell Differentiation / drug effects Cell Differentiation / physiology* Cell Line Mice Osteoblasts / cytology Osteoblasts / metabolism* Pancreatic Polypeptide / genetics Pancreatic Polypeptide / metabolism* Pancreatic Polypeptide / physiology* RNA, Messenger / biosynthesis Receptors, Gastrointestinal Hormone / drug effects Receptors, Gastrointestinal Hormone / metabolism* Reverse Transcriptase Polymerase Chain Reaction
IF 2.843
引用数 11
WOS 分野 PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)