RRC ID |
45302
|
著者 |
Watanuki Y, Kageyama K, Takayasu S, Matsuzaki Y, Iwasaki Y, Daimon M.
|
タイトル |
Ultraviolet B radiation-stimulated urocortin 1 is involved in tyrosinase-related protein 1 production in human melanoma HMV-II cells.
|
ジャーナル |
Peptides
|
Abstract |
Ultraviolet B (UVB) radiation stimulates cutaneous melanin pigmentation. The melanosomal enzyme tyrosinase-related protein 1 (TRP1) is involved in the modulation of pigment production in response to this stressor. Recent molecular and biochemical analyses have revealed the presence of corticotropin-releasing factor (CRF) and urocortin 1 (Ucn1), together with their corresponding receptors, in mammalian skin. Although CRF and Ucn1 are thought to have potent effects on the skin system, their possible roles and regulations have yet to be determined fully. Our previous findings in human melanoma HMV-II cells suggest that both CRF and Ucn1 regulate TRP1 gene expression via Nurr-1/Nur77, transcription factors that constitute the nuclear receptor 4a subgroup of orphan nuclear receptors. HMV-II cells were found to express mainly Ucn1 mRNA. This study aimed to explore the effects of UVB on Ucn1 mRNA and TRP1 protein levels in HMV-II cells. UVB (30 mJ/cm(2)) increased Nurr-1, Nur77, and Ucn1 mRNA levels. UVB also increased TRP1 protein levels. Ucn1 knockdown inhibited the UVB-induced increases in TRP1 protein levels. These data suggest that UVB-stimulated Ucn1 contributes to TRP1 production via the transcription of both Nurr-1 and Nur77. Ucn1, produced in melanoma cells, acts on melanoma cells themselves in an autocrine manner.
|
巻・号 |
61
|
ページ |
93-7
|
公開日 |
2014-11-1
|
DOI |
10.1016/j.peptides.2014.09.009
|
PII |
S0196-9781(14)00277-0
|
PMID |
25240771
|
MeSH |
Autocrine Communication / genetics
Autocrine Communication / radiation effects*
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Humans
Membrane Glycoproteins / biosynthesis*
Membrane Glycoproteins / genetics
Neoplasm Proteins / biosynthesis*
Neoplasm Proteins / genetics
Nuclear Receptor Subfamily 4, Group A, Member 1 / genetics
Nuclear Receptor Subfamily 4, Group A, Member 1 / metabolism
Nuclear Receptor Subfamily 4, Group A, Member 2 / genetics
Nuclear Receptor Subfamily 4, Group A, Member 2 / metabolism
Oxidoreductases / biosynthesis*
Oxidoreductases / genetics
Transcription, Genetic / genetics
Transcription, Genetic / radiation effects*
Ultraviolet Rays*
Urocortins / biosynthesis*
Urocortins / genetics
|
IF |
2.843
|
引用数 |
2
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
HMV-II(RCB0777) |