RRC ID |
12333
|
著者 |
Reinhold H, Soyk S, Simková K, Hostettler C, Marafino J, Mainiero S, Vaughan CK, Monroe JD, Zeeman SC.
|
タイトル |
β-amylase-like proteins function as transcription factors in Arabidopsis, controlling shoot growth and development.
|
ジャーナル |
Plant Cell
|
Abstract |
Plants contain β-amylase-like proteins (BAMs; enzymes usually associated with starch breakdown) present in the nucleus rather than targeted to the chloroplast. They possess BRASSINAZOLE RESISTANT1 (BZR1)-type DNA binding domains--also found in transcription factors mediating brassinosteroid (BR) responses. The two Arabidopsis thaliana BZR1-BAM proteins (BAM7 and BAM8) bind a cis-regulatory element that both contains a G box and resembles a BR-responsive element. In protoplast transactivation assays, these BZR1-BAMs activate gene expression. Structural modeling suggests that the BAM domain's glucan binding cleft is intact, but the recombinant proteins are at least 1000 times less active than chloroplastic β-amylases. Deregulation of BZR1-BAMs (the bam7bam8 double mutant and BAM8-overexpressing plants) causes altered leaf growth and development. Of the genes upregulated in plants overexpressing BAM8 and downregulated in bam7bam8 plants, many carry the cis-regulatory element in their promoters. Many genes that respond to BRs are inversely regulated by BZR1-BAMs. We propose a role for BZR1-BAMs in controlling plant growth and development through crosstalk with BR signaling. Furthermore, we speculate that BZR1-BAMs may transmit metabolic signals by binding a ligand in their BAM domain, although diurnal changes in the concentration of maltose, a candidate ligand produced by chloroplastic β-amylases, do not influence their transcription factor function.
|
巻・号 |
23(4)
|
ページ |
1391-403
|
公開日 |
2011-4-1
|
DOI |
10.1105/tpc.110.081950
|
PII |
tpc.110.081950
|
PMID |
21487098
|
PMC |
PMC3101533
|
MeSH |
Arabidopsis / enzymology*
Arabidopsis / genetics
Arabidopsis / growth & development*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / metabolism*
Base Sequence
Gene Expression Regulation, Plant
Genes, Plant / genetics
Glucans / metabolism
Hydrolysis
Models, Biological
Molecular Sequence Data
Mutant Proteins / isolation & purification
Mutant Proteins / metabolism
Mutation / genetics
Nuclear Proteins / metabolism
Plant Leaves / genetics
Plant Leaves / growth & development
Plant Shoots / enzymology
Plant Shoots / growth & development*
Protein Binding
Protein Structure, Tertiary
Response Elements / genetics
Trans-Activators / metabolism
Transcription Factors / chemistry
Transcription Factors / metabolism*
beta-Amylase / chemistry
beta-Amylase / metabolism*
|
IF |
9.618
|
引用数 |
49
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pdx36441
pdx38715
pdx39980
pdz61236
pdz86189 |