RRC ID 35627
著者 Ito K, Hamasaki K, Kayamori A, Nguyen PA, Amagai K, Wachi M.
タイトル A secondary structure in the 5' untranslated region of adhE mRNA required for RNase G-dependent regulation.
ジャーナル Biosci Biotechnol Biochem
Abstract Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively. Previous research indicates that the 5' untranslated region (5'-UTR) of adhE mRNA gives RNase G-dependency to lacZ mRNA when tagged at the 5'-end, but it has not been elucidated yet how RNase G recognizes adhE mRNA. Primer extension analysis revealed that RNase G cleaved a phosphodiester bond between -19A and -18C in the 5'-UTR (the A of the start codon was defined as +1). Site-directed mutagenesis indicated that RNase G did not recognize the nucleotides at -19 and -18. Random deletion analysis indicated that the sequence from -145 to -125 was required for RNase G-dependent degradation. Secondary structure prediction and further site-directed deletion suggested that the stem-loop structure, with a bubble in the stem, is required for RNaseG-dependent degradation of adhE mRNA.
巻・号 77(12)
ページ 2473-9
公開日 2013-1-1
DOI 10.1271/bbb.130618
PII DN/JST.JSTAGE/bbb/130618
PMID 24317071
MeSH 5' Untranslated Regions / genetics* Alcohol Dehydrogenase / genetics* Aldehyde Oxidoreductases / genetics* Base Sequence Binding Sites Endoribonucleases / metabolism* Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Lac Operon / genetics Nucleic Acid Conformation* Protein Binding
IF 1.516
引用数 6
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME8927(CAG18570)