RRC ID 36261
著者 Iwanami N, Nakamura Y, Satoh T, Liu Z, Satoh AK.
タイトル Rab6 Is Required for Multiple Apical Transport Pathways but Not the Basolateral Transport Pathway in Drosophila Photoreceptors.
ジャーナル PLoS Genet
Abstract Polarized membrane trafficking is essential for the construction and maintenance of multiple plasma membrane domains of cells. Highly polarized Drosophila photoreceptors are an excellent model for studying polarized transport. A single cross-section of Drosophila retina contains many photoreceptors with 3 clearly differentiated plasma membrane domains: a rhabdomere, stalk, and basolateral membrane. Genome-wide high-throughput ethyl methanesulfonate screening followed by precise immunohistochemical analysis identified a mutant with a rare phenotype characterized by a loss of 2 apical transport pathways with normal basolateral transport. Rapid gene identification using whole-genome resequencing and single nucleotide polymorphism mapping identified a nonsense mutation of Rab6 responsible for the apical-specific transport deficiency. Detailed analysis of the trafficking of a major rhabdomere protein Rh1 using blue light-induced chromophore supply identified Rab6 as essential for Rh1 to exit the Golgi units. Rab6 is mostly distributed from the trans-Golgi network to a Golgi-associated Rab11-positive compartment that likely recycles endosomes or transport vesicles going to recycling endosomes. Furthermore, the Rab6 effector, Rich, is required for Rab6 recruitment in the trans-Golgi network. Moreover, a Rich null mutation phenocopies the Rab6 null mutant, indicating that Rich functions as a guanine nucleotide exchange factor for Rab6. The results collectively indicate that Rab6 and Rich are essential for the trans-Golgi network-recycling endosome transport of cargoes destined for 2 apical domains. However, basolateral cargos are sorted and exported from the trans-Golgi network in a Rab6-independent manner.
巻・号 12(2)
ページ e1005828
公開日 2016-2-1
DOI 10.1371/journal.pgen.1005828
PII PGENETICS-D-15-01927
PMID 26890939
PMC PMC4758697
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism Animals Animals, Genetically Modified Drosophila / drug effects Drosophila / genetics Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Endosomes / metabolism Ethyl Methanesulfonate / pharmacology Golgi Apparatus / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Mutagenesis Mutation Photoreceptor Cells, Invertebrate / metabolism* Protein Transport rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism*
IF 5.175
引用数 20
WOS 分野 GENETICS & HEREDITY
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