RRC ID 21141
著者 Tsugama D, Liu H, Liu S, Takano T.
タイトル Arabidopsis heterotrimeric G protein β subunit interacts with a plasma membrane 2C-type protein phosphatase, PP2C52.
ジャーナル Biochim Biophys Acta
Abstract Heterotrimeric G proteins (Gα, Gβ, Gγ) play important roles in signal transduction among various eukaryotic species. G proteins transmit signals by regulating the activities of effector proteins, but only a few Gβ-interacting effectors have been identified in plants. Here we show by a yeast two-hybrid screen that a putative myristoylated 2C-type protein phosphatase, PP2C52, is an Arabidopsis Gβ (AGB1)-interacting partner. The interaction between AGB1 and PP2C52 was confirmed by an in vitro pull-down assay and a bimolecular fluorescence complementation assay. PP2C52 transcripts were detected in many tissues. PP2C52 was localized to the plasma membrane and a mutation in the putative myristoylation site of PP2C52 disrupted its plasma membrane localization. Our results suggest that PP2C52 interacts with AGB1 on the plasma membrane and transmits signals via dephosphorylation of other proteins.
巻・号 1823(12)
ページ 2254-60
公開日 2012-12-1
DOI 10.1016/j.bbamcr.2012.10.001
PII S0167-4889(12)00283-2
PMID 23058975
MeSH Arabidopsis / metabolism* Arabidopsis Proteins / metabolism* Blotting, Western Cell Membrane / metabolism* Fluorescence GTP-Binding Protein beta Subunits / metabolism* GTP-Binding Protein gamma Subunits / metabolism* Genetic Complementation Test Immunoprecipitation Mutation / genetics Phosphoprotein Phosphatases / genetics Phosphoprotein Phosphatases / metabolism* Phosphorylation Protein Phosphatase 2C Protein Processing, Post-Translational RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Subcellular Fractions Two-Hybrid System Techniques
IF 3.411
引用数 18
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda01364 pda10876