RRC ID 6277
著者 Jia F, Gampala SS, Mittal A, Luo Q, Rock CD.
タイトル Cre-lox univector acceptor vectors for functional screening in protoplasts: analysis of Arabidopsis donor cDNAs encoding ABSCISIC ACID INSENSITIVE1-like protein phosphatases.
ジャーナル Plant Mol Biol
Abstract The 14,200 available full length Arabidopsis thaliana cDNAs in the universal plasmid system (UPS) donor vector pUNI51 should be applied broadly and efficiently to leverage a "functional map-space" of homologous plant genes. We have engineered Cre-lox UPS host acceptor vectors (pCR701- 705) with N-terminal epitope tags in frame with the loxH site and downstream from the maize Ubiquitin promoter for use in transient protoplast expression assays and particle bombardment transformation of monocots. As an example of the utility of these vectors, we recombined them with several Arabidopsis cDNAs encoding Ser/Thr protein phosphatase type 2C (PP2Cs) known from genetic studies or predicted by hierarchical clustering meta-analysis to be involved in ABA and stress responses. Our functional results in Zea mays mesophyll protoplasts on ABA-inducible expression effects on the Late Embryogenesis Abundant promoter ProEm:GUS reporter were consistent with predictions and resulted in identification of novel activities of some PP2Cs. Deployment of these vectors can facilitate functional genomics and proteomics and identification of novel gene activities.
巻・号 70(6)
ページ 693-708
公開日 2009-8-1
DOI 10.1007/s11103-009-9502-1
PMID 19499346
PMC PMC2755202
MeSH Abscisic Acid / metabolism Arabidopsis / enzymology* Arabidopsis / genetics* Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism* DNA, Complementary / genetics DNA, Plant / genetics* Gene Expression Profiling Genetic Vectors Phosphoprotein Phosphatases / genetics* Phosphoprotein Phosphatases / metabolism* Phylogeny Plants, Genetically Modified Protein Phosphatase 2C Protoplasts / metabolism Transformation, Genetic Zea mays / genetics Zea mays / metabolism
IF 3.302
引用数 6
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda11685 pda11076 pdy22231 pdz88907 pda19470