RRC ID 47270
著者 Turner HN, Armengol K, Patel AA, Himmel NJ, Sullivan L, Iyer SC, Bhattacharya S, Iyer EPR, Landry C, Galko MJ, Cox DN.
タイトル The TRP Channels Pkd2, NompC, and Trpm Act in Cold-Sensing Neurons to Mediate Unique Aversive Behaviors to Noxious Cold in Drosophila.
ジャーナル Curr Biol
Abstract The basic mechanisms underlying noxious cold perception are not well understood. We developed Drosophila assays for noxious cold responses. Larvae respond to near-freezing temperatures via a mutually exclusive set of singular behaviors-in particular, a full-body contraction (CT). Class III (CIII) multidendritic sensory neurons are specifically activated by cold and optogenetic activation of these neurons elicits CT. Blocking synaptic transmission in CIII neurons inhibits CT. Genetically, the transient receptor potential (TRP) channels Trpm, NompC, and Polycystic kidney disease 2 (Pkd2) are expressed in CIII neurons, where each is required for CT. Misexpression of Pkd2 is sufficient to confer cold responsiveness. The optogenetic activation level of multimodal CIII neurons determines behavioral output, and visualization of neuronal activity supports this conclusion. Coactivation of cold- and heat-responsive sensory neurons suggests that the cold-evoked response circuitry is dominant. Our Drosophila model will enable a sophisticated molecular genetic dissection of cold nociceptive genes and circuits.
巻・号 26(23)
ページ 3116-3128
公開日 2016-12-5
DOI 10.1016/j.cub.2016.09.038
PII S0960-9822(16)31122-8
PMID 27818173
PMC PMC5140760
MeSH Animals Cold Temperature* Drosophila / physiology* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Gene Expression Regulation Larva / physiology Nociception / physiology Sensory Receptor Cells / physiology* Transient Receptor Potential Channels / physiology*
IF 9.601
引用数 25
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
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