RRC ID 49338
著者 Zafar F, Okita AK, Onaka AT, Su J, Katahira Y, Nakayama JI, Takahashi TS, Masukata H, Nakagawa T.
タイトル Regulation of mitotic recombination between DNA repeats in centromeres.
ジャーナル Nucleic Acids Res
Abstract Centromeres that are essential for faithful segregation of chromosomes consist of unique DNA repeats in many eukaryotes. Although recombination is under-represented around centromeres during meiosis, little is known about recombination between centromere repeats in mitotic cells. Here, we compared spontaneous recombination that occurs between ade6B/ade6X inverted repeats integrated at centromere 1 (cen1) or at a non-centromeric ura4 locus in fission yeast. Remarkably, distinct mechanisms of homologous recombination (HR) were observed in centromere and non-centromere regions. Rad51-dependent HR that requires Rad51, Rad54 and Rad52 was predominant in the centromere, whereas Rad51-independent HR that requires Rad52 also occurred in the arm region. Crossovers between inverted repeats (i.e. inversions) were under-represented in the centromere as compared to the arm region. While heterochromatin was dispensable, Mhf1/CENP-S, Mhf2/CENP-X histone-fold proteins and Fml1/FANCM helicase were required to suppress crossovers. Furthermore, Mhf1 and Fml1 were found to prevent gross chromosomal rearrangements mediated by centromere repeats. These data for the first time uncovered the regulation of mitotic recombination between DNA repeats in centromeres and its physiological role in maintaining genome integrity.
巻・号 45(19)
ページ 11222-11235
公開日 2017-11-2
DOI 10.1093/nar/gkx763
PII 4093170
PMID 28977643
PMC PMC5737691
MeSH Centromere / genetics* Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism DNA Helicases / genetics DNA Helicases / metabolism DNA, Fungal / genetics* DNA, Fungal / metabolism Genome, Fungal / genetics Homologous Recombination* Mitosis / genetics* Models, Genetic Rad51 Recombinase / genetics Rad51 Recombinase / metabolism Rad52 DNA Repair and Recombination Protein / genetics Rad52 DNA Repair and Recombination Protein / metabolism Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism
IF 11.502
引用数 9
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