RRC ID 15633
著者 Morita H, Toh H, Fukuda S, Horikawa H, Oshima K, Suzuki T, Murakami M, Hisamatsu S, Kato Y, Takizawa T, Fukuoka H, Yoshimura T, Itoh K, O'Sullivan DJ, McKay LL, Ohno H, Kikuchi J, Masaoka T, Hattori M.
タイトル Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production.
ジャーナル DNA Res
Abstract Lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. The probiotic effects of L. reuteri have been proposed to be largely associated with the production of the broad-spectrum antimicrobial compound reuterin during anaerobic metabolism of glycerol. We determined the complete genome sequences of the reuterin-producing L. reuteri JCM 1112(T) and its closely related species Lactobacillus fermentum IFO 3956. Both are in the same phylogenetic group within the genus Lactobacillus. Comparative genome analysis revealed that L. reuteri JCM 1112(T) has a unique cluster of 58 genes for the biosynthesis of reuterin and cobalamin (vitamin B(12)). The 58-gene cluster has a lower GC content and is apparently inserted into the conserved region, suggesting that the cluster represents a genomic island acquired from an anomalous source. Two-dimensional nuclear magnetic resonance (2D-NMR) with (13)C(3)-glycerol demonstrated that L. reuteri JCM 1112(T) could convert glycerol to reuterin in vivo, substantiating the potential of L. reuteri JCM 1112(T) to produce reuterin in the intestine. Given that glycerol is shown to be naturally present in feces, the acquired ability to produce reuterin and cobalamin is an adaptive evolutionary response that likely contributes to the probiotic properties of L. reuteri.
巻・号 15(3)
ページ 151-61
公開日 2008-6-30
DOI 10.1093/dnares/dsn009
PII dsn009
PMID 18487258
PMC PMC2650639
MeSH Chromosome Mapping Genome, Bacterial Genomic Islands* Glyceraldehyde / analogs & derivatives* Glyceraldehyde / metabolism Lactobacillus fermentum / genetics* Lactobacillus fermentum / metabolism Lactobacillus reuteri / genetics* Lactobacillus reuteri / metabolism Metabolic Networks and Pathways / genetics Models, Biological Multigene Family Phylogeny Propane / metabolism* Vitamin B 12 / biosynthesis* Vitamin B 12 / genetics
IF 4.009
引用数 153
WOS 分野 GENETICS & HEREDITY
リソース情報
一般微生物 JCM 1112