RRC ID 27769
著者 Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.
タイトル Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome.
ジャーナル DNA Res
Abstract To understand the epigenetic regulation required for germ cell-specific gene expression in the mouse, we analysed DNA methylation profiles of developing germ cells using a microarray-based assay adapted for a small number of cells. The analysis revealed differentially methylated sites between cell types tested. Here, we focused on a group of genomic sequences hypomethylated specifically in germline cells as candidate regions involved in the epigenetic regulation of germline gene expression. These hypomethylated sequences tend to be clustered, forming large (10 kb to ~9 Mb) genomic domains, particularly on the X chromosome of male germ cells. Most of these regions, designated here as large hypomethylated domains (LoDs), correspond to segmentally duplicated regions that contain gene families showing germ cell- or testis-specific expression, including cancer testis antigen genes. We found an inverse correlation between DNA methylation level and expression of genes in these domains. Most LoDs appear to be enriched with H3 lysine 9 dimethylation, usually regarded as a repressive histone modification, although some LoD genes can be expressed in male germ cells. It thus appears that such a unique epigenomic state associated with the LoDs may constitute a basis for the specific expression of genes contained in these genomic domains.
巻・号 20(6)
ページ 549-65
公開日 2013-12-1
DOI 10.1093/dnares/dst030
PII dst030
PMID 23861320
PMC PMC3859323
MeSH Animals Carcinogenesis / genetics Cell Line DNA Methylation* Embryonic Stem Cells / metabolism Epigenesis, Genetic Female Gene Duplication Gene Expression Profiling Germ Cells / metabolism* Histones / metabolism Male Mice Mice, Transgenic Molecular Sequence Data Neoplasm Proteins / genetics Nuclear Proteins / genetics Segmental Duplications, Genomic Testis / metabolism X Chromosome / genetics X Chromosome / metabolism*
IF 4.009
引用数 9
WOS 分野 GENETICS & HEREDITY
リソース情報
実験動物マウス RBRC00209 RBRC00821 RBRC00822
ヒト・動物細胞 GS-DG1(AES0002)