RRC ID 6459
著者 Manickavelu A, Kawaura K, Oishi K, Shin-I T, Kohara Y, Yahiaoui N, Keller B, Suzuki A, Yano K, Ogihara Y.
タイトル Comparative gene expression analysis of susceptible and resistant near-isogenic lines in common wheat infected by Puccinia triticina.
ジャーナル DNA Res
Abstract Gene expression after leaf rust infection was compared in near-isogenic wheat lines differing in the Lr10 leaf rust resistance gene. RNA from susceptible and resistant plants was used for cDNA library construction. In total, 55 008 ESTs were sequenced from the two libraries, then combined and assembled into 14 268 unigenes for further analysis. Of these ESTs, 89% encoded proteins similar to (E value of < or =10(-5)) characterized or annotated proteins from the NCBI non-redundant database representing diverse molecular functions, cellular localization and biological processes based on gene ontology classification. Further, the unigenes were classified into susceptible and resistant classes based on the EST members assembled from the respective libraries. Several genes from the resistant sample (14-3-3 protein, wali5 protein, actin-depolymerization factor and ADP-ribosylation factor) and the susceptible sample (brown plant hopper resistance protein, caffeic acid O-methyltransferase, pathogenesis-related protein and senescence-associated protein) were selected and their differential expression in the resistant and susceptible samples collected at different time points after leaf rust infection was confirmed by RT-PCR analysis. The molecular pathogenicity of leaf rust in wheat was studied and the EST data generated made a foundation for future studies.
巻・号 17(4)
ページ 211-22
公開日 2010-8-1
DOI 10.1093/dnares/dsq009
PII dsq009
PMID 20360266
PMC PMC2920755
MeSH Basidiomycota / genetics Basidiomycota / pathogenicity* Expressed Sequence Tags* Gene Expression Profiling / methods* Gene Expression Regulation, Fungal Gene Expression Regulation, Plant Genes, Plant* Molecular Sequence Data Plant Diseases / genetics Plant Diseases / microbiology Plant Leaves / genetics Plant Leaves / microbiology Triticum / genetics* Triticum / microbiology*
IF 4.009
引用数 43
WOS 分野 GENETICS & HEREDITY
リソース情報
コムギ CJ272808-CJ919993