RRC ID 44276
著者 Isshiki M, Ohta H, Tamura H.
タイトル Coffee reduces SULT1E1 expression in human colon carcinoma Caco-2 cells.
ジャーナル Biol Pharm Bull
Abstract Recent epidemiological studies have shown that moderate coffee consumption is associated with a lower risk of certain types of cancers, particularly colon cancer in postmenopausal women. To elucidate the molecular basis for the preventive action of coffee, we investigated the effect of coffee on estrogen sulfotransferase (SULT) because sulfation is the major pathway involved in the inactivation of estrogens. We found that coffee reduced SULT1E1 gene expression in human colon carcinoma Caco-2 cells. Treatment with 2.5% (v/v) coffee for 24 h resulted in a 60% reduction of the expression of the SULT1E1 gene in Caco-2 cells. Corresponding to reduced SULT1E1 gene expression, cytosolic estrogen SULT activity toward E(2) (20 nM) decreased by 25%. In addition, an accumulation of E(2) sulfates in the medium, which reflects cellular activity of estrogen SULT, decreased after the cells were treated with coffee. Major bioactive constituents in coffee such as caffeine, chlorogenic acid and caffeic acid did not show any effect. The inhibitory activity was extractable by using ethyl acetate. We also found that the inhibitory activity was produced by roasting the coffee beans. Mithramycin, an inhibitor of the transcription factor stimulating protein 1 (Sp1), was able to restore coffee-reduced SULT1E1 gene expression. Our data suggest that daily coffee consumption may reduce estrogen SULT activity, thereby enhancing estrogenic activity in the colon.
巻・号 36(2)
ページ 299-304
公開日 2013-1-1
DOI 10.1248/bpb.b12-00902
PII DN/JST.JSTAGE/bpb/b12-00902
PMID 23370358
MeSH Caco-2 Cells Cell Survival / drug effects Coffea* Coffee Colonic Neoplasms / enzymology Estradiol / metabolism Gene Expression Regulation, Enzymologic / drug effects* Humans Nucleic Acid Synthesis Inhibitors / pharmacology Plant Extracts / pharmacology* Plicamycin / pharmacology Protein Synthesis Inhibitors / pharmacology Sp1 Transcription Factor / antagonists & inhibitors Sulfotransferases / genetics* Sulfotransferases / metabolism
IF 1.863
引用数 7
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 CACO-2(RCB0988)