RRC ID 52677
著者 Fukamachi M, Kasamatsu A, Endo-Sakamoto Y, Fushimi K, Kasama H, Iyoda M, Minakawa Y, Shiiba M, Tanzawa H, Uzawa K.
タイトル Multiple coagulation factor deficiency protein 2 as a crucial component in metastasis of human oral cancer.
ジャーナル Exp Cell Res
Abstract Multiple coagulation factor deficiency protein 2 (MCFD2), a binding partner of lectin mannose binding 1 (LMAN1), causes combined deficiencies of coagulation factors V and VIII. MCFD2 function in inherited hematologic disorders is well elucidated; however, little is known about its role in human tumorigenesis. The aim of the current study was to investigate the states of MCFD2 in oral squamous cell carcinoma (OSCC). The expression of MCFD2 was up-regulated significantly in all cell lines examined. Evaluation of the cellular functions associated with tumoral metastasis showed that MCFD2 knockdown (shMCFD2) cells exhibited significantly lower cellular invasiveness and migration and higher cellular adhesion compared with shControl cells. Of note, shMCFD2 cells also showed weak immunoreactivity of LMAN1 and a lower secretion level of galactoside-binding soluble 3 binding protein (LGALS3BP). In addition to in vitro validation, clinical data on 70 patients with OSCC indicated that state of MCFD2 expression level is associated with regional lymph node metastasis. Altogether, we have demonstrated that MCFD2 promotes cancer metastasis by regulating LMAN1 and LGALS3BP expression levels. Hence, MCFD2 may represent a promising candidate for a novel therapeutic target for patients with metastatic OSCCs.
巻・号 368(1)
ページ 119-125
公開日 2018-7-1
DOI 10.1016/j.yexcr.2018.04.021
PII S0014-4827(18)30241-6
PMID 29679592
MeSH Antigens, Neoplasm / genetics* Biomarkers, Tumor / genetics* Calcium / metabolism Carcinoma, Squamous Cell / genetics Carrier Proteins / genetics* Glycoproteins / genetics* Humans Mannose-Binding Lectins / genetics* Membrane Proteins / genetics* Mouth Neoplasms / genetics* Mutation, Missense / genetics* Neoplasm Metastasis / genetics* Vesicular Transport Proteins / genetics*
IF 3.383
引用数 6
リソース情報
ヒト・動物細胞 Ca9-22(RCB1976) HO-1-u-1(RCB2102) SAS(RCB1974)