RRC ID 32393
著者 Fairchild MJ, Smendziuk CM, Tanentzapf G.
タイトル A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis.
ジャーナル Development
Abstract Interactions between the soma and germline are essential for gametogenesis. In the Drosophila testis, differentiating germ cells are encapsulated by two somatic cells that surround the germline throughout spermatogenesis. chickadee (chic), the fly ortholog of Profilin, mediates soma-germline interactions. Knockdown of Chic in the soma results in sterility and severely disrupted spermatogenesis due to defective encapsulation. To study this defect further, we developed a permeability assay to analyze whether the germline is isolated from the surrounding environment by the soma. We find that germline encapsulation by the soma is, by itself, insufficient for the formation of a permeability barrier, but that such a barrier gradually develops during early spermatogenesis. Thus, germline stem cells, gonialblasts and early spermatogonia are not isolated from the outside environment. By late spermatocyte stages, however, a permeability barrier is formed by the soma. Furthermore, we find that, concomitant with formation of the permeability barrier, septate junction markers are expressed in the soma and localize to junctional sites connecting the two somatic cells that surround the germline. Importantly, knockdown of septate junction components also disrupts the permeability barrier. Finally, we show that germline differentiation is delayed when the permeability barrier is compromised. We propose that the permeability barrier around the germline serves an important regulatory function during spermatogenesis by shaping the signaling events that take place between the soma and the germline.
巻・号 142(2)
ページ 268-81
公開日 2015-1-15
DOI 10.1242/dev.114967
PII dev.114967
PMID 25503408
MeSH Animals Cell Differentiation / physiology* Cellular Microenvironment / physiology* Drosophila / embryology* Drosophila Proteins / metabolism* Germ Cells / growth & development* Male Permeability Profilins / metabolism* Spermatogenesis / physiology*
IF 5.611
引用数 22
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 9553R-3