RRC ID 49398
著者 Hirota K, Okamoto T, Matsuyama H, Yumoto I.
タイトル Polygonibacillus indicireducens gen. nov., sp. nov., an indigo-reducing and obligate alkaliphile isolated from indigo fermentation liquor for dyeing.
ジャーナル Int J Syst Evol Microbiol
Abstract Obligately alkaliphilic and halophilic strains, designated In2-9T and D2-7, were isolated from a fermented Polygonum indigo (Polygonum tinctorium Lour.) liquor sample obtained from a craft centre in Date City, Hokkaido, Japan. The 16S rRNA gene sequence phylogeny suggested that strain In2-9T is a member of the genus Bacillus with the closest relatives being the alkaliphilic species of the genus Bacillus, Bacillus hemicellulosilyticusJCM 9152T (96.4 % 16S rRNA gene sequence similarity) and Bacillus alcalophilus DSM 485T (96.5 %). Cells of the isolate stained Gram-positive and were facultatively anaerobic straight rods that were motile by peritrichous flagella. Strain In2-9T grew between 13 and 45 °C with optimum growth at approximately 35-37 °C. The isolates grew in the pH range of 8-12 with optimum growth at pH 10. The isoprenoid quinone detected was menaquinone-6 (MK-6) and the DNA G+C content was 39.4 mol%. The whole-cell fatty acid profile mainly (>10 %) consisted of iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. Spore shape and location and chemotaxonomic characteristics revealed that the isolates were distinctly different from phylogenetic neighbouring alkaliphilic species of the genus Bacillus. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, the isolates represent a novel species of a new genus, for which the name Polygonibacillusindicireducens gen. nov., sp. nov. is proposed. The type strain of the type species is In2-9T (=JCM 30831T=NCIMB 14982T), and strain D2-7 is an additional strain of the species.
巻・号 66(11)
ページ 4650-4656
公開日 2016-11-1
DOI 10.1099/ijsem.0.001405
PMID 27503611
MeSH Bacillaceae / classification* Bacillaceae / genetics Bacillaceae / isolation & purification Bacterial Typing Techniques Base Composition Coloring Agents DNA, Bacterial / genetics Fatty Acids / chemistry Fermentation* Indigo Carmine Japan Nucleic Acid Hybridization Phylogeny* Polygonum / microbiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.415
引用数 7
リソース情報
一般微生物 JCM 30831 JCM 9152 JCM 5262