RRC ID 602
著者 Fujimoto S, Yonemura M, Matsunaga S, Nakagawa T, Uchiyama S, Fukui K.
タイトル Characterization and dynamic analysis of Arabidopsis condensin subunits, AtCAP-H and AtCAP-H2.
ジャーナル Planta
Abstract Condensin complexes are thought to play essential roles in mitotic chromosome assembly and segregation in eukaryotes. To date, two condensin complexes (condensin I and II) have been identified. Both complexes contain two structural maintenance of chromosome (SMC) subunits and three non-SMC subunits. In plants, little is known about the localization and function of all the condensin subunits. Here, we report the analyses on the localization of a non-SMC subunit of Arabidopsis condensin I and II, AtCAP-H, and AtCAP-H2, respectively. Our study indicated that localization of AtCAP-H and AtCAP-H2 is dynamically changed through the mitotic cell cycle using GFP-tagged AtCAP-H and AtCAP-H2 in tobacco cultured cells. They are localized at mitotic chromosomes from prometaphase to telophase. However, their localization in interphase is quite different. AtCAP-H was mainly found in the cytoplasm whereas AtCAP-H2 was mainly found in a nucleolus. It is revealed using GFP-tagged deletion mutant s of AtCAP-H that the kleisin-gamma middle domain (GM domain) is a unique domain only in AtCAP-H, responsible for chromosomal localization. We propose that the GM domain of CAP-H is essential for its chromosomal localization at mitosis and thus proper function of CAP-H. Differences in localization of AtCAP-H and AtCAP-H2 at interphase also suggest their functional differentiation.
巻・号 222(2)
ページ 293-300
公開日 2005-10-1
DOI 10.1007/s00425-005-1546-0
PMID 15883832
MeSH Adenosine Triphosphatases / chemistry Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Amino Acid Sequence Arabidopsis / cytology Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cells, Cultured Chromosomal Proteins, Non-Histone / chemistry Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Chromosomes, Plant / metabolism Cloning, Molecular DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Flowers / genetics Flowers / metabolism Gene Expression Regulation, Plant Mitosis Molecular Sequence Data Multiprotein Complexes / chemistry Multiprotein Complexes / genetics Multiprotein Complexes / metabolism* Plants, Genetically Modified Protein Structure, Tertiary Protein Subunits / chemistry Protein Subunits / genetics Protein Subunits / metabolism Protein Transport Sequence Homology, Amino Acid Tobacco / genetics Tobacco / metabolism
IF 3.39
引用数 18
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00008