RRC ID 35639
著者 Ge X, Lyu ZX, Liu Y, Wang R, Zhao XS, Fu X, Chang Z.
タイトル Identification of FkpA as a key quality control factor for the biogenesis of outer membrane proteins under heat shock conditions.
ジャーナル J Bacteriol
Abstract The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp double-deletion strain of E. coli, although lethal and defective in the biogenesis of OMPs at the normal growth temperature, is viable and effective at the heat shock temperature. We identified FkpA as the multicopy suppressor for the lethal phenotype of the ΔsurA Δskp strain. We also demonstrated that the deletion of fkpA from the ΔsurA cells resulted in only a mild decrease in the levels of folded OMPs at the normal temperature but a severe decrease as well as lethality at the heat shock temperature, whereas the deletion of fkpA from the Δskp cells had no detectable effect on OMP biogenesis at either temperature. These results strongly suggest a functional redundancy between FkpA and SurA for OMP biogenesis under heat shock stress conditions. Mechanistically, we found that FkpA becomes a more efficient chaperone for OMPs under the heat shock condition, with increases in both binding rate and affinity. In light of these observations and earlier reports, we propose a temperature-responsive OMP biogenesis mechanism in which the degrees of functional importance of the three chaperones are such that SurA > Skp > FkpA at the normal temperature but FkpA ≥ SurA > Skp at the heat shock temperature.
巻・号 196(3)
ページ 672-80
公開日 2014-2-1
DOI 10.1128/JB.01069-13
PII JB.01069-13
PMID 24272780
PMC PMC3911151
MeSH Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Deletion Gene Expression Regulation, Bacterial / physiology Genotype Hot Temperature* Kinetics Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Chaperones / genetics Molecular Chaperones / metabolism Peptidylprolyl Isomerase / genetics Peptidylprolyl Isomerase / metabolism*
IF 3.006
引用数 20
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME9062(BW25113) JW0052-KC JW3309-KC