RRC ID |
11401
|
著者 |
Nishimura K, Ashida H, Ogawa T, Yokota A.
|
タイトル |
A DEAD box protein is required for formation of a hidden break in Arabidopsis chloroplast 23S rRNA.
|
ジャーナル |
Plant J
|
Abstract |
In plant chloroplasts, the ribosomal RNA (rRNA) of the large subunit of the ribosome undergoes post-maturation fragmentation processing. This processing consists of site-specific cleavage that generates gapped, discontinuous rRNA molecules. However, the molecular mechanism underlying introduction of the gap structure (the 'hidden break') is poorly understood. Here, we found that the DEAD box protein RH39 plays a key role in introduction of the hidden break into the 23S rRNA in Arabidopsis chloroplasts. Genetic screening for an Arabidopsis plant with a drastically reduced level of ribulose-1,5-bisphosphate carboxylase/oxygenase identified an RH39 mutant. The levels of other chloroplast-encoded photosynthetic proteins were also severely reduced. The reductions were not due to a failure of transcription, but rather inefficiency in translation. RNA gel blotting revealed incomplete fragmentation of 23S rRNA in chloroplasts during maturation. In vitro analysis with recombinant RH39 suggested that the protein binds to the adjacent sequence upstream of the hidden break site to exert its function. We propose a molecular mechanism for the RH39-mediated fragmentation processing of 23S rRNA in chloroplasts.
|
巻・号 |
63(5)
|
ページ |
766-77
|
公開日 |
2010-9-1
|
DOI |
10.1111/j.1365-313X.2010.04276.x
|
PII |
TPJ4276
|
PMID |
20561259
|
MeSH |
Amino Acid Sequence
Arabidopsis / genetics*
Arabidopsis / metabolism
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Blotting, Northern
Blotting, Western
Chloroplasts / metabolism
DEAD-box RNA Helicases / genetics*
DEAD-box RNA Helicases / metabolism
Genes, Essential / genetics
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Molecular Sequence Data
Mutation
Protein Binding
RNA, Chloroplast / genetics
RNA, Chloroplast / metabolism
RNA, Ribosomal, 23S / genetics*
RNA, Ribosomal, 23S / metabolism
Recombinant Proteins / metabolism
Ribulose-Bisphosphate Carboxylase / genetics
Ribulose-Bisphosphate Carboxylase / metabolism
Sequence Homology, Amino Acid
|
IF |
6.141
|
引用数 |
51
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda13720 |