論文 - 詳細
RRC ID | 50312 |
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著者 | Sasakura Y, Yoshida K, Treen N. |
タイトル | Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease. |
ジャーナル | Methods Mol Biol |
Abstract |
The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods. |
巻・号 | 1630 |
ページ | 235-245 |
公開日 | 2017-1-1 |
DOI | 10.1007/978-1-4939-7128-2_19 |
PMID | 28643263 |
MeSH | Animals Ciona intestinalis / genetics* Electroporation Gene Editing / methods* Gene Knockout Techniques Organ Specificity Transcription Activator-Like Effector Nucleases / metabolism |
リソース情報 | |
カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |